复方钩藤降压片对SHR心肌IL-6及MMP-9表达的影响  

Effect of Compound Uncaria Jiangya Tablet on Expressions of IL-6 and MMP-9 in SHR Myocardium

作　　者：曾勇[1] 谭超[1] 唐陆军 谭元生[1] 文爱珍 ZENG Yong;TAN Chao;TANG Lujun;TAN Yuanshen;WEN Aizhen(Hunan University of Chinese Medicine,Changsba 410007,Hunan,China)

机构地区：[1]湖南中医药大学

出　　处：《中华中医药学刊》2018年第10期2358-2361,I0009-I0011,共7页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81473616);教育部重点实验室开放基金中医内科学省部共建项目(ZYNK201507);湖南省中医药科研计划重点项目(201534);湖南省教育厅重点项目(15A142)

摘　　要：目的：试从干预SHR心肌局部白细胞介素-6（IL-6）及基质金属蛋白酶-9（MMP-9）表达的角度,完善复方钩藤降压片干预逆转高血压左室肥厚的机制。方法：将50只14周龄雄性SHR随机分为5组,即复方钩藤降压片组高剂量组（H组）、中剂量组（M组）、低剂量组（L组）、贝那普利组（B组）、SHR空白对照组（S组）,另10只Wistar大鼠空白对照组（W组）,相应处理干预24周,后取材HE染色法观察心肌组织形态学、免疫组化法检测心肌局部IL-6及MMP-9表达水平。结果：（1）对心肌形态学的影响：H组大部分大鼠心肌细胞形态正常,无明显细胞水肿及间质纤维变性或坏死; M组少部分心肌细胞轻度水肿或轻度间质纤维变性,个别组心肌间质出现炎症反应; L组部分大鼠心肌水肿较明显,个别大鼠心肌细胞坏死、间质水肿或纤维变性; B组大部分心肌细胞明显水肿,部分心肌间质水肿或纤维变性; S组大部分心肌细胞水肿,细胞体积明显增大,心肌间质水肿或纤维变性（图1-E）; W组各组心肌细胞形态正常,间质无水肿等表现;（2） IL-6：H组、M组、L组、B组及W组之间两两比较表达量均无明显区别,均无统计学意义（P〉 0. 05）,H组、M组、L组及W组表达量均低于S组,差异具有统计学意义（P 〈0. 05）,但B组与S组比较,表达量无明显差异（P 〈0. 05）;（3） MMP-9：H组、M组、L组、B组及W组之间两两比较表达量均无明显区别,均无统计学意义（P〉 0. 05）,H组及M组表达量均低于S组,差异具有统计学意义（P 〈0. 05）,L组、B组及W组与S组相比无统计学意义（P〉 0. 05）。结论：SHR心肌大部分心肌细胞水肿、体积明显增大,心肌间质水肿或纤维变性,局部炎症水平升高,但局部MMP-9表达量无明显升高。复方钩藤降压片有一定的保护心肌和干预逆转高血压LVH的作用,其机制与降低心脏局部炎症水平有关。Objective： To study the mechanism of Compound Uncaria Jiangya Tablet reversing the hypertensive left ventricular hypertrophy by interfering the expressions of regional IL-6 and MMP-9 in SHR myocardium. Methods： Fifty male SHRs,14 weeks old,were randomly divided into 5 groups,namely Compound Uncaria Hypotensive Tablet group and high dose group（ H group）,middle dose group（ M group）,low dose group（ L group）,Benner Pury group（ B group）,SHR control group（ S group）. The other 10 Wistar rats were as the control group（ group W）. The corresponding treatment intervention lasted for 24 weeks. HE was used to observe myocardial tissue morphology. The immunohistochemistry was used to test myocardial MMP-9 and IL-6 expressions. Results：（1） The effects on myocardial morphology：In group H,the rats myocardial cells were normal and there was no obvious cell edema,interstitial fibrous degeneration or necrosis of myocardial cells and there was a few mild edema or mild interstitial fibrosis,myocardial interstitial inflammatory response. L group＇s rats myocardial edema was obvious and there was necrosis myocardial cells in interstitial edema or fibrosis. B group＇s myocardial cell had edema,myocardial interstitial edema or fibrosis. S group＇s myocardial cell had edema,increased volume,myocardial interstitial edema or fibrosis（ Figure 1-E）. W group＇s myocardial cell morphology was normal,with no interstitial edema.（2） IL-6：H group,M group,L group,B group and W group showed no significant difference when comparing with each other（ P〉0. 05）. H group,M group,L group and W group＇s expression was lower than that of S group,and the difference was statistically significant（ P〈0. 05）,but the B group＇s had no significant difference compared with that of S group（ P〈0. 05）.（3） MMP-9：H group,M group,L group,B group and W group＇s expression had no obvious difference when comparing with each other（ P〉0. 05）. H group and M group＇s expressions were lower than tho

关 键 词：复方钩藤降压片 自发性高血压大鼠 白细胞介素-6 基质金属蛋白酶-9 左室肥厚 

分 类 号：R285.5[医药卫生—中药学]