电针内关预处理对心肌缺血再灌注大鼠模型血管内皮因子及线粒体功能影响  

Effects of Electroacupuncture at Neiguan Preconditioning on Vascular Endothelial Factor and Mitochondrial Function in Rats with Myocardial Ischemia Reperfusion Injury

作　　者：韩永丽 严江天 吴松 陈松 王华 梁凤霞 黄伟 周焕娇 HAN Yongli;YAN Jiangtian;WU Song;CHEN Song;WANG Hua;LIANG Fengxia;HUANG Wei;ZHOU Huanjiao(College of Acupuncture-Moxibustion and Orthpaedics,Hubei University of TCM,Wuhan 430061,Hubei,China;Hubei Collaborative Innovation Center,Acupuncture and Moxibustion for Disease Prevention,Wahan 430061,Hubei,China)

机构地区：[1]湖北中医药大学针灸骨伤学院,湖北武汉430061 [2]湖北中医药大学针灸骨伤学院、针灸治未病湖北省协同创新中心,湖北武汉430061

出　　处：《中华中医药学刊》2018年第10期2389-2393,I0015,共6页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金青年科学基金项目(81403460);湖北中医药大学教育教学研究项目(2014A06)

摘　　要：目的：观察电针＂内关＂预处理对心肌缺血再灌注大鼠氧化应激及线粒体功能的影响。并从p38MAPK信号通路的角度分析其作用机制。方法：将50只大鼠随机分为5组：假手术组、模型组、电针预处理组、抑制剂预处理组和电针＋抑制剂预处理组。进行相应组电针内关穴,治疗结束后,测定大鼠心肌ROS、ICAM-1、VCAM-1、ATP和RCR的含量。结果：与S组比较,M组、EA组、SB组及EA＋SB组大鼠心肌细胞ICAM-1、VCAM-1蛋白表达含量明显下降,RCR及ATP含量明显下降（P 〈0. 05）,SB组、EA组及EA＋SB组ATP含量及ICAM-1、VCAM-1与M组比较,均有显著性差异（P 〈0. 05）,其中EA组各项指标优于EA＋SB组及SB组（P 〈0. 05）。结论：I/R中炎症反应增强,电针内关预处理可以抑制大鼠I/R模型中心肌细胞黏附分子ICAM-1I/R和VCAM-1的蛋白表达,能激活内源性的抗炎因子,发挥保护受损心肌细胞的效应,并有效缓解钙超载,减轻胞内Ca^2＋负荷。Objective： To observe the effect of electroacupuncture at Neiguan（ PC6） preconditioning on oxidative stress and mitochondrial function in rats with myocardial ischemia and reperfusion. And the mechanism of action was analyzed from the perspective of p38 MAPK signal pathway. Methods： Fifty rats were randomly divided into five groups： sham operation group,model group,electroacupuncture pretreatment group,inhibitor pretreatment group and electroacupuncture ＋inhibitor pretreatment group. The levels of ROS,ICAM-1,VCAM-1,ATP and RCR in myocardium were measured at the end of the treatment. Results： Compared with S group,the expressions of ICAM-1 and VCAM-1 protein in M group,EA group,SB group and EA ＋ SB group decreased significantly,and the contents of RCR and ATP decreased significantly（ P〈0. 05）. The EA group and EA ＋ SB group＇s were significantly higher than those of EA ＋ SB group and SB（P〈0. 05）,and there was significant difference between IC group and EA group（P〈0. 05）. Conclusion： I/R inflammatory response increases. Electroacupuncture at Neiguan（ PC6） pretreatment can inhibit the I/R rats＇ myocardial cell adhesion molecules ICAM-1 I/R and VCAM-1 protein expression,activate endogenous anti-inflammatory factors,play a role in protecting damaged cardiomyocytes,effectively relieve calcium overload,and reduce intracellular Ca^2＋load.

关 键 词：电针 预处理 缺血再灌注 线粒体 炎性因子 血管内皮 

分 类 号：R245.97[医药卫生—针灸推拿学]