三七茜草复方有效组分宫内缓释系统调控大鼠子宫内膜形态学及出凝血机制的研究  

Impact of Effective Components of Notoginseng Madder Compound of Levonorgestrel-Releasing Intrauterine System on Endometrial Morphology of Rats and Blood Coagulation Mechanism

作　　者：张芳[1] 杨蕾 李盼盼[1] 李彦楠[1] 徐丽[2] 师伟[3] 李澄 王领弟 孙孟瑶 于航[1] ZHANG Fang;YAN Lei;LI Panpan;LI Yannan;XU Li;SHI Wei;LI Cheng;WANG Lingdi;SUN Mengyao;YU Hang(Shandong University of Chinese Medicine,Jinan 250014,Shandong,China;Provincial Hospital affiliated to Shandong University,Jinan 250021,Shandong,China;The affiliated Hospital of Shandong University of Chinese Medicine,Jinan 250014,Shandong,China)

机构地区：[1]山东中医药大学,山东济南250014 [2]山东大学附属省立医院,山东济南250021 [3]山东中医药大学附属医院,山东济南250014

出　　处：《中华中医药学刊》2018年第10期2407-2410,I0019,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81674014,81001536);山东省自然科学基金项目(ZR2016HP30);山东省人口和计划生育委员会科技计划项目(2013年第11号)

摘　　要：目的：探讨三七茜草复方有效组分（SQ）宫内缓释系统调控大鼠子宫内膜出凝血机制。方法：选定32只SD雌性大鼠,随机分为SQ-IUD组、IUD模型组、缓释材料（LR）对照组、空白对照组,观察大鼠子宫大体形态学、子宫内膜形态学,ELISA检测宫腔冲洗液内前列环素（PGI2）、血栓烷（TXA2）、纤溶酶原激活物抑制剂（PAI-1）、组织型纤溶酶原激活剂（t-PA）的含量。结果：在子宫大体形态学方面,SQ-IUD组充血水肿和渗出粘连程度较IUD模型组明显减轻;在子宫内膜形态学方面,SQ-IUD组上皮细胞脱落、腺体扩张、间质水肿、炎性细胞浸润程度较IUD模型组明显减轻;在PAI-1、t-PA方面,SQ-IUD组PAI-1的含量较IUD模型组明显升高,SQIUD组、IUD模型组PAI-1的含量分别为（205. 45±16. 03） pg/m L、（177. 22±22. 08） pg/m L（P 〈0. 05）,SQ-IUD组t-PA的含量较IUD模型组明显降低,SQ-IUD组、IUD模型组t-PA的含量分别为（3. 20±0. 29） ng/m L、（3. 61±0. 47） ng/m L（P 〈0. 05）;在TXA2、PGI2方面,SQ-IUD组二者的含量较IUD模型组均明显升高,SQIUD组、IUD模型组TXA2的含量分别为：（260. 88±23. 40） pg/m L、（228. 68±31. 31） pg/m L（P 〈0. 05）,SQ-IUD组、IUD模型组的PGI2含量分别为：（235. 25±23. 59） pg/m L、（200. 88±22. 90） pg/m L（P 〈0. 05）;在TXA2/PGI2方面,SQ-IUD组的比值较IUD模型组略低,SQ-IUD组、IUD模型组的TXA2/PGI2比值含量分别为：（1. 12±0. 15）、（1. 14±0. 14）（P〉 0. 05）。结论：三七茜草缓释系统可明显减轻IUD放置后子宫炎症反应,改善子宫内膜形态;三七复方有效成分可能通过调节t-PA、PAI-1和TXA2、PGI2平衡而起到防治IUD出血不良反应。Objective：To explore the effective components of notoginseng madder compound（ SQ） of Levonorgestrel-releasing intrauterine system on endometrial morphology of rats and blood coagulation mechanism. Methods：Thirty-two SD female rats were randomly divided into SQ-IUD group,IUD model group,LR control group and blank control group.We observed rats＇ uterus morphology and endometrial morphology. ELISA was used to detect the contents of propion（PGI2）,thrombutane（TXA2）,plasminogen activator inhibitor（PAI-1） and tissue plasminogen activator（t-pa） in the uterine washing fluid. Results：In the general morphology of the uterus,the level of congestion,edema and adhesion of SQ-IUD group were obviously lower than those of the IUD model group. In terms of endometrial morphology,the level of epithelial cell shedding,glands expansion,interstitial edema and inflammatory cells infiltration of SQ-IUD group were obviously lower than those of IUD model group. In terms of PAI-1 and t-PA,the contents of PAI-1 of SQ-IUD group were obviously higher than those of IUD model. The contents of PAI-1 of SQ-IUD group and IUD model were（205. 45 ± 16. 03）pg/m L,（177. 22 ± 22. 08）pg/m L respectively（P〈0. 05）. The content of t-PA of SQ-IUD group was obviously lower than that of IUD model. The contents of t-PA of SQ-IUD group and IUD model were（3. 20 ±0. 29） ng/m L and（3. 61 ± 0. 47） ng/m L respectively（ P〈0. 05）. In terms of TXA2 and PGI2,the contents of TXA2 and PGI2 of SQ-IUD group were obviously higher than those of IUD model. The contents of TXA2 of SQ-IUD group and IUD model were（260. 88 ± 23. 40） pg/m L and（228. 68 ± 31. 31） pg/m L,respectively（ P〈0. 05）. The contents of PGI2 of SQ-IUD group and IUD model were（235. 25 ± 23. 59） pg/m L and（200. 88 ± 22. 90） pg/m L,respectively（ P〈0. 05）. In terms of TXA2/PGI2,SQ-IUD group＇s was slightly lower than that of IUD model. The ratio of TXA2/PGI2 in SQ-IUD group and IUD model group was 1. 12 ± 0. 15 and 1. 14 ±

关 键 词：三七茜草复方有效组分 宫内缓释系统 子宫内膜形态学 出凝血机制 

分 类 号：R285.5[医药卫生—中药学]