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作 者:符显昭[1] 冯雪萍[1] 黄文华 李春燕 李星婵 丘海先[1] 王清礼 班富度 FU Xianzhao;FENG Xueping;HUANG Wenhua;LI Chunyan;LI Xingchan;QIU Haixian;WANG Qingli;BAN Fudu(Clinic Medical College of Youjiang Medieal National College,Baise 533099,Guangxi,China)
机构地区:[1]右江民族医学院临床医学院
出 处:《中华中医药学刊》2018年第10期2423-2427,I0026-I0029,共9页Chinese Archives of Traditional Chinese Medicine
基 金:国家自然科学基金地区项目(81460698);广西自然科学基金项目(2015 GXNSFAA139221);广西重点研发计划项目(2017AB45042);广西中医药民族医药传承创新专项项目(GZLC16-51)
摘 要:目的:探讨降糖舒心方(JTSX)抑制SD糖尿病大鼠心肌细胞内质网应激c-JNK凋亡旁路,减轻糖尿病心肌重构的分子机制。方法:采用高脂饲养+链脲佐菌素制备2型糖尿病大鼠模型,成模后随机分为模型组、西药组、JTSX低剂量组(JTSX1组)和JTSX高剂量组(JTSX2组),另设正常组作对照。相应药物干预8周后,检测空腹血糖(FBG)、血脂、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)变化。用透射电镜观察心肌组织超微结构; TUNEL法检测心肌细胞凋亡水平;用RT-PCR和免疫组化分别检测c-JUN氨酸端激酶(c-JNK)和糖调节蛋白78(GRP78)的mRNA转录及其功能蛋白的表达水平。结果:与模型组相比,各给药组均能明显降低FBG(P 〈0. 05),升高SOD、GSH-Px(P 〈0. 05);均可降低TG、LDL-C(P 〈0. 05),升高HDL-C(P 〈0. 05);均可下调c-JNK、GRP78mRNA转录及其蛋白表达(P 〈0. 05),降低心肌细胞凋亡指数(AI)(P 〈0. 05),并改善心肌组织超微结构;与西药组相比,JTSX2疗效更显著(P 〈0. 05)。结论:JTSX能改善糖尿病代谢紊乱,提高抗氧化应激能力,抑制内质网应激细胞凋亡通路,从而改善心肌重构,疗效具有剂量依赖性。Objecive:To investigate the molecular mechanism of Jiangtang Shuxin( JTSX) Recipe inhibiting endoplasmic reticulum stress c-JNK apoptotic pathway and reducing myocardial remodeling in SD diabetic rats. Methods: Type 2 diabetes were established in male SD rats by injecting streptozotocin and feeding high lipid diet. After T2 DM models established successfully,the rats were divided into model group,Western medicine group( Gliquidone + Benazepril),low-dose JTSX group( JTSX1) and high-dose JTSXgroup(JTSX2). Another normal group was used as control group. After 8 weeks of corresponding drug intervention,the levels of fasting blood glucose( FBG),serum superoxide dismutase( SOD),glutathione peroxidase( GSH-Px) were measured. The ultrastructure of myocardial tissue was observed with transmission electron microscope. TUNEL was used to detect cardiomyocyte apoptosis. The mRNA and their functional protein levels of c-Jun N-terminal kinases( c-JNK) and glucose-regulated protein78( GRP78) were determined by the method of RT-PCR and immunohistochemistry respectively. Results:Compared with model group,after drugs intervention,all administered groups could significantly decrease FPG( P〈0. 05),elevate SOD and GSH-Px activity( P〈0. 05); all could depress the levels of TG and LDL-C( P〈0. 05),and raise HDL-C( P〈0. 05);all could cut down the mRNA transcription and their functional protein expression of the c-JNK and GRP78( P〈0. 05); all could reduce cardiomyocyte apoptosis index( AI)( P〈0. 05) and improve the ultrastructure of myocardial tissue. Compared with Western medicine group,the JTSX2 effect was more significant( P〈0. 05). Conclusion:JTSX can improve metabolic disorders in diabetes mellitus,ameliorate the ability of antioxidant stress,suppress endoplasmic reticulum stress apoptosis c-JNK pathway,and therefore improve myocardial remodeling,and the therapeutic effect was dose-dependent.
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