黄芪的毒理学试验研究  

Study on the toxicity of astragalus membranaceus

作　　者：胡志航[1] 赵建斌 柴建新 刘臻[1] 刘冬英[1] 王茵[1] HU Zhi-hang;ZHAO Jian-bin;CHAI Jian-xin;LIU Zhen;LIU Dong-ying;WANG Yin(Institute of Health Food,Zhejiang Academy of Medical Sciences,Hangzhou,Zhejiang 310013,China)

机构地区：[1]浙江省医学科学院保健食品研究所,浙江杭州310013 [2]山西振东制药股份有限公司,山西长治046000

出　　处：《中国卫生检验杂志》2018年第18期2206-2210,共5页Chinese Journal of Health Laboratory Technology

基　　金：山西省重点研发计划重点项目(201603D3114015);山西省黄芪资源产业化及产业国际化协同创新中心项目(HQXTCXZX2016-018);浙江省医学支撑学科营养学(11-zc03);浙江省科技厅项目(2017F30001)

摘　　要：目的通过试验观察黄芪的急性经口毒性、遗传毒性以及亚慢性毒性,为进一步开发利用黄芪提供了科学依据。方法对黄芪进行急性经口毒性测定;通过细菌回复突变试验、哺乳动物红细胞微核试验及小鼠精母细胞染色体畸变试验对其遗传毒性进行评价;对大鼠进行90 d经口毒性试验,通过血液学指标、血生化指标、尿常规指标、食物利用率及脏体比等指标对其亚慢性毒性进行评价。结果黄芪的急性经口毒性LD50>62.0 g/kg BW,3项遗传毒性试验均为阴性,亚慢性毒性试验各项指标均为阴性。结论黄芪在此次试验条件下未见急性毒性、遗传毒性以及亚慢性毒性。Objective To determine the acute oral toxicity,genetic toxicity and subchronic toxicity of astragalus membranaceus,so to provide experimental basis for its application. Methods Acute oral toxicity test was performed in mice. The genetic toxicity was evaluated by bacterial reverse mutation assay,micronuclear test of mammalian and chromosome aberration test of spermatocyte in mice. 90-day oral toxicity test was performed in rat,and the result was evaluated by hematological index,serum biochemical index,routine urine test,food utility rate and organ-body ratios. Results The acute oral LD50 of astragalus membranaceus was greater than 62. 0 g/kg BW. Bacterial reverse mutation assay,micronuclear test of mammalian and chromosom aberration test of spermatocyte in mice and 90-day oral toxicity test showed negative results. Conclusion Under the present condition,astragalus membranaceus was non-acute oral toxicity,non-genetic toxicity and non-subchronic toxicity.

关 键 词：黄芪 急性经口毒性 遗传毒性 亚慢性毒性试验 

分 类 号：R285[医药卫生—中药学]