槲皮素对人成骨肉瘤MG-63细胞体外增殖与凋亡的影响  

Effect of quercetin on in vitro proliferation and apoptosis of human osteosarcoma MG-63 cells

作　　者：孙南阳[1] 符芳姿 谭江波 SUN Nan-yang;FU Fang-zi;TAN Jiang-bo(The Second Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410005,Hunan,China;Haikou Hospital Affiliated to Xiangya School of Medicine,Central South University,Haikou 570208,Hainan,China;Xiangdong Hospital Affiliated to Hunan Normal University,Zhuzhou 412200,China)

机构地区：[1]湖南中医药大学第二附属医院,湖南长沙410005 [2]中南大学湘雅医学院附属海口医院,海南海口570208 [3]湖南师范大学附属湘东医院,湖南株洲412200

出　　处：《湖南中医杂志》2018年第9期158-160,共3页Hunan Journal of Traditional Chinese Medicine

摘　　要：目的:观察槲皮素对人成骨肉瘤MG-63细胞增殖和凋亡的影响。方法:不同浓度的槲皮素作用于体外培养的MG-63细胞,四甲基偶氮唑盐(MTT)法检测槲皮素对MG-63细胞增殖的抑制能力,流式细胞术(FCM)检测槲皮素对MG-63细胞凋亡的影响。结果:槲皮素可显著抑制MG-63细胞增殖,具有明显剂量和时间依赖性;槲皮素可明显诱导MG-63细胞凋亡。结论:槲皮素能够抑制MG-63细胞增殖,诱导MG-63细胞凋亡。Objective： To investigate the effect of quercetin on in vitro proliferation and apoptosis of human osteosarcoma MG-63 cells. Methods： Different concentrations of quercetin were used for in vitro culture of MG-63 cells. MTT assay was used to assess the inhibitory effect of quercetin on the proliferation of MG-63 cells,and flow cytometry was used to evaluate the effect of quercetin on the apoptosis of MG-63 cells. Results： Quercetin significantly inhibited the proliferation of MG-63 cells in a dose-and time-dependent manner. It a lso induced the apoptosis of MG-63 cells. Conclusion： Quercetin can inhibit the proliferation of MG-63 cells and induce the apoptosis of MG-63 cells.

关 键 词：槲皮素 人成骨肉瘤MG-63细胞 细胞增殖 细胞凋亡 实验研究 

分 类 号：R285.5[医药卫生—中药学]