紫外-可见分光光度法测定多花黄精多糖含量  

Content determination of polysaccharides in Polygonatum cyrtonema Hua by ultraviolet-visible spectrophotometry

作　　者：曾婷[1,2,3] 周芳 汤嫣然[1,2,3] 袁汉文 刘永蓓 李亚霖 唐巧凤 彭彩云 王炜[1,2,3] ZENG Ting;ZHOU Fang;TANG Yan-ran;YUAN Han-wen;LIU Yong-bei;LI Ya-lin;TANG Qiao-feng;PENG Cai-yun;WANG Wei(Laboratory of Traditional Chinese Medicine ＆ Folk Medicine Innovation and International Development,Hunan University of Chinese Medicine,Changsha 410208,Hunan,China;China-Pakistan Traditional Chinese Medicine ＆ Folk Medicine International Cooperation Base,Changsha 410208,Hunan,China;Innovative Drug Research Institute,School of Pharmacy,Hunan University of Chinese Medicine,Changsha,410208,Hunan,China)

机构地区：[1]湖南中医药大学中药民族药物创新发展国际联合实验室,湖南长沙410208 [2]中国-巴基斯坦中医药民族医药研究国际合作基地,湖南长沙410208 [3]湖南中医药大学药学院创新药物研究所,湖南长沙410208

出　　处：《湖南中医杂志》2018年第9期167-169,共3页Hunan Journal of Traditional Chinese Medicine

基　　金：湖南省科技厅重点研发项目(编号:2018SK2119);国家中医药管理局国家中药标准化项目-黄精标准化建设(编号:ZYBZH-Y-HUN-23);湖南省长沙市科技计划项目(编号:Kq1706055);湖南省研究生创新性试验计划项目(编号:CX2017B443);湖南省大学生创新性试验计划项目(编号:201713);湖南中医药大学助研计划项目(编号:201716)

摘　　要：目的:测定湖南洪江国家黄精标准化基地多花黄精中的多糖含量,为多花黄精的进一步开发利用及药材质量标准研究奠定基础。方法:以葡萄糖为标准品,采用硫酸-蒽酮试剂显色,应用紫外-可见分光光度法测定多花黄精多糖含量。结果:葡萄糖在3.28~19.68μg/m L范围内线性良好(r=0.9992),多花黄精多糖平均含量为8.05%。结论:洪江种植基地的多花黄精品质优良,洪江是道地黄精药材种植理想基地。Objective： To investigate the method for content determination of polysaccharides in Polygonatum cyrtonema Hua from Hunan Hongjiang National Polygonatum sibiricum Standardized Base,and to lay a foundation for further development and utilization of Polygonatum cyrtonema Hua and research on quality standards of medicinal materials. Methods： With glucose as the standard substance,the sulfuric acid-anthrone method and ultraviolet-visible spectrophotometry were used to determine the content of polysaccharides in Polygonatum cyrtonema Hua. Results： Glucose showed a good linear relationship within the range of 3. 28 - 19. 68μg/m L（ r = 0. 9992）,and the average content of polysaccharides in Polygonatum cyrtonema Hua was 8. 05%. Conclusion：Polygonatum cyrtonema Hua from the Hongjiang plantation base has good quality,and Hongjiang is an ideal base for the plantation of genuine Polygonatum sibiricum medicinal material.

关 键 词：多花黄精 黄精多糖 含量测定 

分 类 号：R284.1[医药卫生—中药学]