碱性高锰酸钾分光光度法测定盐酸肼屈嗪含量  

Determination of Hydralazine Hydrochloride by Alkaline Potassium Permanganate Spectrophotometry

作　　者：牟城健 王振 许钟元 马卫兴 李树安 MOU Cheng-jian;WANG Zhen;XU Zhong-yuan;MA Wei-xing;LI Shu-an(College of Chemical Engineering;College of Pharmacy,Huaihai Institute of Technology,Lianyungang 222005,China)

机构地区：[1]淮海工学院化学工程学院,江苏连云港222005 [2]淮海工学院药学院,江苏连云港222005

出　　处：《化学试剂》2018年第10期981-984,共4页Chemical Reagents

基　　金：连云港市科技局产业前瞻与共性关键技术项目(CG1613);连云港市工业攻关项目(CG1508);国家海洋公益性行业科研专项项目(201305007);淮海工学院博士启动基金项目(KQ16001);淮海工学院大学生创新创业项目(Z201711641104003);江苏省六大人才高峰资助项目(2009年、2013年);江苏省海洋资源开发研究院科研项目(JSIMR201409);连云港市微博双创项目(2017)

摘　　要：基于盐酸肼屈嗪在碱性条件与高锰酸钾发生氧化还原显色反应,建立了碱性高锰酸钾分光光度法测定盐酸肼屈嗪的新方法,详细研究了该体系的反应条件。实验发现最大显色波长分别在420 nm和600 nm处,最大褪色波长在530 nm处,在此三波长处测定的表观摩尔吸光系数分别是1.566×106、3.40×106和2.43×106L/（mol·cm）,盐酸肼屈嗪浓度均在0.01～0.07 mg/L范围内,吸光度与浓度呈现线性关系,其线性回归方程分别是A=7.903 6C＋0.030 9、A=12.368C＋0.062 4和A=17.311C-0.144 1,相关系数R分别是0.999 5、0.999 8和0.999 4,测得的相对标准偏差（RSD）均小于±5%,回收率均在98%~102%之间。所拟方法成功地用于盐酸肼屈嗪片剂中盐酸肼屈嗪的含量测定,结果与硼酸催化茚三酮法所得结果一致。Based on the redox chromogenic reaction of hydralazine hydrochloride with potassium permanganate in alkaline conditions,a new method of alkaline potassium permanganate spectrophotometric determination of hydralazine hydrochloride was established.The reaction conditions of the system were investigated in detail.The maximum color developing wavelength was at 420 nm and 600 nm,respectively,and the maximum fading wavelength was at 530 nm.The apparent molar absorptivities measured at these three wavelengths were 1. 566×106 L/（mol·cm）,3. 40 × 106 L/（mol·cm） and 2. 43 × 106 L/（mol·cm）,respectively. Hydrazine hydralazine concentration in the range of 0. 01 ~ 0. 07 mg/L,absorbance and concentration showed a linear relationship.The linear regression equation were A = 7. 903 6 C＋0. 030 9,A = 12. 368 C＋0. 062 4 and A = 17. 311 C-0. 144 1,the correlation coefficients R were 0. 999 5,0. 999 8 and 0. 999 4,respectively,and the relative standard deviations were all less than 5%.The recoveries were were all 98% ～ 102%.The proposed method was successfully applied to the determination of hydralazine hydrochloride in hydralazine hydrochloride tablets.The results were consistent with that of boric acid catalytic ninhydrin method.

关 键 词：盐酸肼屈嗪 高锰酸钾 分光光度法 显色反应 

分 类 号：O657.32[理学—分析化学]