一例禽腺病毒感染的病例诊断  

Diagnosis of a Case of Avian Adenovirus Infection

作　　者：史荣华 颜彩霞 张建军 任丹 高巍[2] SHI Rong-hua;YAN Cai-xia;ZHANG Jian-jun(Sinopharm Yangzhou VAC Biological Engineering Co.Ltd.,Yangzhou,Jiangsu 225127)

机构地区：[1]国药集团扬州威克生物工程有限公司,江苏扬州225127 [2]扬州大学兽医学院,江苏扬州225009

出　　处：《安徽农业科学》2018年第30期86-88,共3页Journal of Anhui Agricultural Sciences

摘　　要：2018年4月,山东省某肉鸡场发生某种疾病,日死亡率达5%,临床剖检主要病变为心包积液,出血性肝炎,肾脏肿大。为了明确患病肉鸡死亡的原因及确定病原,无菌采取肝脏、肾脏等病变组织,开展了病毒分离,PCR检测和组织病理学观察等实验室诊断。结果表明,病料接种鸡胚,鸡胚有明显的肝出血症状,PCR扩增产物测序结果与已发表的血清4型禽腺病毒Hexom基因同源性为99%,组织病理学观察发现肝细胞核内形成嗜碱性包涵体。上述结果表明该分离株为血清4型禽腺病毒。In April 2018, a certain disease occurred in a broiler farm in Shandong Province. The daily mortality rate was 5%. The main clinical manifestations were pericardial effusion, hemorrhagic hepatitis and kidney enlargement. In order to find out the cause of death and determine the pathogen of broiler disease, aseptic liver, kidney and other pathological tissues were used to conduct laboratory diagnosis of virus isolation, PCR detection and histopathology. Chicken embryos inoculated with the diseased material showed obvious hepatorrhagic symptoms. The sequencing results of PCR amplified products showed 99% homology with the published serotype 4 avian adenovirus Hexom gene. Histopathological observation showed that basophilic inclusion bodies were formed in the nuclei of the liver. The above results indicated that the isolate was serum type 4 FAdV.

关 键 词：肉鸡 腺病毒 诊断 

分 类 号：S855.3[农业科学—临床兽医学]