H_2O_2预处理转pepc基因水稻的生理生化特性  

Regulation of Hydrogen Peroxide Pre-Treatment on Physiological Characteristics of pepc Transgenic Rice

作　　者：魏晓东[1] 李霞[1] 唐玉婷[1] WEI Xiao-dong;LI Xia;TANG Yu-ting(Jiangsu High Quality Rice Research and Development Center,Institute of Food Crops,Jiangsu Academy of Agricuhural Sciences,Nanjing 210014,China)

机构地区：[1]江苏省农业科学院粮食作物研究所江苏省优质水稻工程技术研究中心,南京210014

出　　处：《淮阴工学院学报》2018年第5期25-30,共6页Journal of Huaiyin Institute of Technology

基　　金：江苏省自然科学基金项目(BK20130708);江苏省自主创新基金项目(CX[(13)5002])

摘　　要：以稳定遗传的转pepc基因水稻(PC)和未转基因的原种Kitaake(WT)为实验材料,研究模拟干旱条件下(12%PEG6000干旱处理)WT和PC花期剑叶的光合气体交换参数和活性氧代谢,用H_2O_2进行预处理(PEG6000+0.5 mmol/L H_2O_2),探究H_2O_2预处理对模拟干旱下转pepc基因水稻的调节作用。结果表明,模拟干旱降低2个品种的净光合速率和气孔导度,提高水分利用效率,同时两者光合速率的差距进一步加大,PC水稻光合优势更加明显。H_2O_2预处理后,2个品种的净光合速率均有回升;PC水稻叶片气孔导度高于WT,使其在模拟干旱下具有较高的光合速率,从而减轻干旱对光合作用的抑制。H_2O_2预处理降低了WT在模拟干旱下的SOD、CAT、APX活性,也降低了PC水稻的SOD、APX活性。The stable heredity pepc transgenic rice （PC） and nontransgenic rice Kitaake＂ （WT） were used to study photosynthetic gas exchange parameters and reactive oxygen species metabolism of flag leaves at flowering stage in two varieties under simulated drought （12% PEG6000 drought treatment）. With hydrogen peroxide （H202） pretreatment （12% PEG6000 ＋ 0.5 mmol/LH202）, the modulation of H202 pretreatment to pepc transgenic rice under simulated drought were investigated. The results showed that simulated drought led to the decrease of net photosynthetic rates and stomatal conductance, and the increase of water use efficiency in two varieties. The difference between PC and WT became larger, and photosynthetic superiority of PC was more evi- dent. After H202 pretreatment, net photosynthetic rates of two varieties rebounded under simulated drought. The stomatal conductance of PC was higher than WT, which attributed to higher photosynthetic rate of transgenic plants, and could alleviate the inhibition of photosynthesis of drought stress. H202 pre -treatment reduced SOD, CAT and APX activities in WT, and decreased SOD and APX in PC under simulated drought.

关 键 词：水稻 模拟干旱 磷酸烯醇式丙酮酸羧化酶 过氧化氢 光合特性 抗氧化酶 

分 类 号：S311[农业科学—作物栽培与耕作技术] S184[农业科学—农艺学]