过表达miR-155对斑马鱼胚胎发育的影响  

Effects of miR-155 overexpression on the development of zebrafish embryos

作　　者：黄梦圆 李德利[2] 张加良[1] 袁栎[2] 王晖[1] Huang Mengyuan;Li Deli;Zhang Jialiang;Yuan Li;Wang Hui(Department of Cardiology,the First Affiliated Hospital of NMU,Nanjing 210029;Department of Biochemistry and Molecular Biology,NMU,Nanjing 211166,China)

机构地区：[1]南京医科大学第一附属医院心内科,江苏南京210029 [2]南京医科大学生物化学与分子生物学系,江苏南京211166

出　　处：《南京医科大学学报（自然科学版）》2018年第8期1028-1033,共6页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省自然基金(BK2012648);江苏省六大人才高峰(2015-WSN-033);江苏省高等学校自然科学研究重大项目(17KJA310001)

摘　　要：目的:探讨过表达mi R-155对斑马鱼胚胎发育的影响和机制。方法:利用q RT-PCR检测mi R-155在斑马鱼胚胎发育各阶段的表达;通过显微注射的方法将mi R-155 mimic转入斑马鱼胚胎,实现基因的过表达;利用q RT-PCR和Western blot方法检测过表达mi R-155后发育相关标志性基因的表达变化;利用ETS1的m RNA对过表达mi R-155的胚胎进行补救实验。结果:mi R-155在胚胎发育时期均有表达,在器官形成期表达量明显增高;过表达mi R-155导致斑马鱼胚胎发育迟缓、褪膜延迟、腹部及心包积液、尾部变短变粗,肝脏、小肠、心脏及肌肉发育相关标志性基因表达下调;ETS1能缓解过表达mi R-155所致发育畸形。结论:过表达mi R-155可能通过负向调控ETS1而影响斑马鱼胚胎肝脏、小肠、心脏及肌肉的发育。Objective：To explore the role and mechanism of mi R-155 overexpression in the development of zebrafish embryos.Methods：The expression of mi R-155 during each stage of embryogenesis was detected by quantitative RT-PCR. mi R-155 mimic was microinjected to the 4 blastomeres of 4-cell stage embyos to overexpress the gene. The expressions of developmental related marker genes were detected by quantitative RT-PCR,and ETS1 m RNA was used to rescue embryos after overexpression of mi R-155. Results：The expression of mi R-155 was found in the whole embryonic development period and obviously increased in the periods of organogenesis. Overexpression of mi R-155 leads to retardation of embryo development,delayed membrane depletion,abdominal and pericardial effusion,tail shortening and thickening,and down regulated the expression of genes related to liver,intestine,heart and muscle development. ETS1 can reduce the developmental malformation caused by overexpression of mi R-155. Conclusion：Overexpression of mi R-155 may affect embryonic development of zebrafish through negative regulation of ETS1.

关 键 词：MIR-155 ETS1 斑马鱼 胚胎发育 

分 类 号：Q344[生物学—遗传学]