长链非编码RNA NANCI-NKX2.1信号通路在BPD新生小鼠肺组织中作用的研究  

The role of long noncoding RNA NANCI-NKX2.1 signaling pathway in lung tissue of neonatal mice with bronchopulmonary dysplasia

作　　者：王秀桂[1] 王会芳 张媛[1] 田兆方[1] Wang Xiugui;Wang Huifang;Zhang Yuan;Tian Zhaofang(Department of Neonatology,Huai＇an First Hospital,NM;the Pediatric Diagnosis and Treatment Respiratory Key Laboratory of Huai＇an,Huaian 223300,China)

机构地区：[1]南京医科大学附属淮安第一医院新生儿科,淮安市小儿呼吸诊疗重点实验室,江苏淮安223300

出　　处：《南京医科大学学报（自然科学版）》2018年第8期1070-1076,共7页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省临床医学专项(BL2014063);淮安市创新平台(HAP2016007)

摘　　要：目的:探讨长链非编码RNA(lnc RNA)NANCI-NKX2.1信号通路在新生小鼠支气管肺发育不良(bronchopulmonary dysplasia,BPD)形成过程中的作用。方法:将32只C57BL/6J新生小鼠随机分为实验组、阴性对照组、生理盐水对照组和空白对照组,每组8只。实验组于生后第2天经鼻腔吸入含lnc RNA NANCI干扰序列的腺病毒Ad-lnc NANCI sh RNA的生理盐水1μL,阴性对照组将含无关序列发卡结构病毒质粒Ad-GFP NC的生理盐水1μL导入肺内,生理盐水对照组将生理盐水1μL导入肺内,空白对照组除未予鼻腔吸入以外其他控制因素与上3组相同;于出生后7 d处死各组小鼠并采集肺组织。采用HE染色法察看小鼠肺组织病理变化,采用RT-q PCR检测NANCI表达量,RT-q PCR及Western blot技术分别检测NKX2.1、SPC、AQP5 m RNA和蛋白表达水平。结果:实验组肺组织病理示新隔形成减少,肺泡腔扩大、部分融合,肺泡数目较少,RAC值下降(P<0.05);肺组织NANCI表达量下降(P<0.05);NKX2.1、SPC及AQP5 m RNA和蛋白的表达量亦下降(P<0.05)。结论:NANCI-NKX2.1信号通路在BPD新生小鼠中可能起重要作用。Objective：To investigate the role of NANCI-NKX2.1 signaling pathway in the process of bronchopulmonary dysplasia（BPD） formation in neonatal mice. Methods：A total of 32 neonatal mice（C57 BL/6 J） were randomly divided into 4 groups：interference group,negative control group,normal saline control group and blank control group,with 8 pups in each group. One microlitre of normal saline with lnc RNA NANCI adenovirus and control adenovirus were administered intranasally to lungs of newborn mice in interference group and negative control group at postnatal days 2 respectively. One microlitre of normal saline was administered intranasally to lungs of newborn mice in normal saline control group.No interference treatment was processed in blank control group.The mice were sacrificed 7 days after birth and lung tissue samples were collected. Hematoxylin-eosin（HE）staining was used to observe pathological changes in lung tissues. RT-q PCR was used to measure the expression of NANCI. RT-q PCR and Western blot were used to measure the m RNA and protein expression of NKX2.1,SPC and AQP5. Results：HE staining in interference group showed thinner alveolar septum,increased alveolar cavity and decreased number of alveoli and septum. Lung tissue biopsy also showed decreased RAC values（P〈0.05）.The relative expression of NANCI decreased（P〈0.05）;the expression of NKX2.1,SPC and AQP5 m RNA and protein were decreased（P〈0.05）. Conclusion：NANCI-NKX2.1 signaling pathway may play an important role in BPD neonatal mice.

关 键 词：长链非编码RNA NANCI NKX2.1 SPC AQP5 

分 类 号：R722.11[医药卫生—儿科]