隔药饼灸对慢性支气管炎模型大鼠肺组织TNF-α蛋白及其mRNA表达的影响  

Effect of Herbal Cake-Separated Moxibustion on the Expression of TNF-α Protein and Its mRNA in Lung Tissue of Chronic Bronchitis Model Rats

作　　者：孙璐 谭静[1] 阳仁达[1] 言芳[1] 廖宗力 魏星 罗明 SUN- Lu, TAN Jing, YANG Renda, YAN Fang, LIAO Zongli, WEI Xing, LUO Ming(Hunan University of Chinese Medicine, Changsha, Hunan 410208, China)

机构地区：[1]湖南中医药大学,湖南长沙410208

出　　处：《湖南中医药大学学报》2018年第11期1273-1277,共5页Journal of Hunan University of Chinese Medicine

基　　金：湖南省教育厅创新平台项目(16K068);湖南省大学生创新实验项目(017-132)

摘　　要：目的观察隔药饼灸对慢性支气管炎大鼠肺组织中肿瘤坏死因子-α(TNF-α)蛋白及其mRNA含量的影响。方法将48只SD大鼠随机分为空白组、模型组、隔药饼灸组、隔纸垫灸组,每组12只。采取改良烟熏法复制慢性支气管炎大鼠模型,观察用隔药饼灸治疗后大鼠的一般情况,并与空白组、模型组、隔纸垫灸组进行对照,大鼠肺组织中的TNF-αmRNA的表达用RT-PCR法检测,肺组织中TNF-α的蛋白表达用Western blot法检测。结果与模型组相比,隔药饼组和隔纸垫组大鼠肺及气管的病理损伤均有好转;隔药饼灸组和隔纸垫灸组都能使慢支大鼠肺组织TNF-αmRNA及TNF-α蛋白含量表达降低(P<0.05),且隔药饼灸组效果更好(P<0.05)。结论隔药饼灸能改善慢支大鼠一般症状及气道炎症,提示其机制可能是通过下调TNF-α蛋白及其mRNA的表达达到治疗慢性支气管炎的目的。Objective To observe the ettect of herbal cake-separated moxibustion on the content of tumor necrosis factor-α （TNF-α） protein and its mRNA in the lung tissue of rats with chronic bronchitis （CB）. Methods Forty-eight Sprague-Dawley rats were randomly and equally divided into tour groups, including blank group, model group, herbal cake-separated moxibustion group, and paper pad-separated moxibustion group. CB model rats were prepared by modified tumigation. The general status of rats after herbal cake-separated moxibustion was observed and compared with that in the other three groups. The expression of TNF- α mRNA in rat lung was measured by RT-PCR and the protein expression of TNF-α in lung tissues was measurred by Western blot Results The pathological injury- of lung and trachea of rats in herbal cake separated moxibustion group and paper pad- separated moxibustion group was improved compared with that in the model group. Herbal cake-eparated moxibustion and paper pad- separated moxibustion can reduce the expression of TNF-α mRNA and TNF-α protein in the lung tissues of CB rats （P〈0.05） and the ettect of herbal cake-eparated moxibustion was better （P〈0.05） Conclusion Herbal cake-eparated moxibustion can improve the general symptoms and airway inflammation of CB rats, possibly by down-regulating the expression of TNF-α protein and its mRNA.

关 键 词：慢性支气管炎 隔药饼灸 肿瘤坏死因子Α 肿瘤坏死因子α信使RNA 

分 类 号：R256.1[医药卫生—中医内科学] R245.8[医药卫生—中医学]