Comparative study of brine shrimp bioassay-based toxic activities of three harmful microalgal species that frequently blooming in aquaculture ponds  

作　　者：YANG Xiaoqian WEN Xin ZHOU Chengxu ZHU Xiaojuan MENG Ran LUO Qijun YAN Xiaojun 杨小倩;文欣;周成旭;朱小娟;孟冉;骆其君;严小军(School of Marine Science,Ningbo University;Senior High School of Yudu;Key Laboratory of Marine Biotechnology of Zhejiang Province,Ningbo University;Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center,Ningbo University)

机构地区：[1]School of Marine Science,Ningbo University,Ningbo 315211,China [2]Key Laboratory of Marine Biotechnology of Zhejiang Province,Ningbo University,Ningbo 315211,China [3]Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center,Ningbo University,Ningbo 315211,China [4]Senior High School of Yudu,Ganzhou 343200,China

出　　处：《Journal of Oceanology and Limnology》2018年第5期1697-1706,共10页海洋湖沼学报（英文）

基　　金：Supported by the Earmarked Fund for Modern Agro-industry Technology Research System,China(No.CARS-49);the Ningbo Science and Technology Research Projects,China(No.2017C110003);the Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Development Fund;the National 111 Project of China;the K.C.Wong Magna Fund of Ningbo University

摘　　要：Typical harmful micro-algal species constantly occurred in high density in marine aquaculture ponds in Xiangshan and Sanmen Bay, Zhejiang Province. Fates of the microalgal cells influenced by activity of the cultured animals largely determined the ef fects of the harmful microalgae. However, it is difficult to detect the in situ process. In this paper, toxic activities of three harmful microalga, namely P rymnesium parvum, Pleurochrysis elongata, Karlodinium veneficum, which were isolated from the local ponds, were comparatively studied based on brine shrimp toxic bioassays. Diff erent lethal activities of live cells, cell debris, cellular extracts, and cell free mediums prepared by different process were analyzed. The results showed that,(1) all of the three microalgal species had density and time dependent lethal ef fects on A rtermia nauplii, while P. parvum was the most toxic one and had acute lethal eff ects in 5 h. No such acute lethal eff ects were observed in P. elongata or K. veneficum;(2) live cells, cell debris and cellular extracts of P. parvum had the same lethal pattern. Prymnesins, toxin from P. parvum, is probably not exotoxic active; For P. elongata, toxic activity mainly came from live cells and cell debris; For K. veneficum, toxic activity was relatively lower compared with the other two species. However, Karlotoxin, toxin from K. veneficum, is exotoxic active. Physical disturbance triggered K. veneficum cells actively releasing toxins, which made it an active predator.Typical harmful micro-algal species constantly occurred in high density in marine aquaculture ponds in Xiangshan and Sanmen Bay, Zhejiang Province. Fates of the microalgal cells influenced by activity of the cultured animals largely determined the ef fects of the harmful microalgae. However, it is difficult to detect the in situ process. In this paper, toxic activities of three harmful microalga, namely P rymnesium parvum, Pleurochrysis elongata, Karlodinium veneficum, which were isolated from the local ponds, were comparatively studied based on brine shrimp toxic bioassays. Diff erent lethal activities of live cells, cell debris, cellular extracts, and cell free mediums prepared by different process were analyzed. The results showed that,（1） all of the three microalgal species had density and time dependent lethal ef fects on A rtermia nauplii, while P. parvum was the most toxic one and had acute lethal eff ects in 5 h. No such acute lethal eff ects were observed in P. elongata or K. veneficum;（2） live cells, cell debris and cellular extracts of P. parvum had the same lethal pattern. Prymnesins, toxin from P. parvum, is probably not exotoxic active; For P. elongata, toxic activity mainly came from live cells and cell debris; For K. veneficum, toxic activity was relatively lower compared with the other two species. However, Karlotoxin, toxin from K. veneficum, is exotoxic active. Physical disturbance triggered K. veneficum cells actively releasing toxins, which made it an active predator.

关 键 词：Prymnesium parvum Pleurochrysis elongata Karlodinium veneficum Artemia nauplii toxic activity 

分 类 号：S967[农业科学—水产养殖] Q949.206[农业科学—水产科学]