双丹明目胶囊对糖尿病大鼠模型视网膜VEGF家族表达的影响  

Effects of Shuangdan Mingmu capsule on the expressions of VEGF in the retina of diabetic rat models

作　　者：彭俊[1] 潘坤[2] 刘峥嵘[2] 秦裕辉[1,2] 彭清华[1,2] Jun Peng;Kun Pan;Zheng-Rong Liu;Yu-HuiQin;Qing-Hua Peng(Key Disciplines of Chinese Medicine Ophthalmology,HunanUniversity of Traditional Chinese Medicine,Changsha 410208,Hunan Province,China;Key Laboratory of Otorhinolaryngology of Traditional Chinese medicine,Hunan University of TraditionalChinese Medicine,Changsha 410208,Hunan Province,China)

机构地区：[1]湖南中医药大学中医眼科学重点学科,中国湖南省长沙市410208 [2]湖南中医药大学中医五官科湖南省重点实验室,中国湖南省长沙市410208

出　　处：《国际眼科杂志》2018年第11期1958-1962,共5页International Eye Science

基　　金：国家自然科学基金面上项目(No.81473737);湖南省研究生科研创新项目(No.CX2016B377;CX2017B432);中医药防治五官科疾病湖南省重点实验室建设项目(No.2017TP1018);长沙市科技计划项目(No.kc1704005);中央财政支持地方高校建设项目;国家中医药管理局中医眼科学重点学科建设项目;湖南省中医五官科学重点学科建设项目~~

摘　　要：目的:观察双丹明目胶囊对糖尿病大鼠模型视网膜VEGF家族因子VEGF-a、VEGF-b、VEGF-c蛋白表达的影响。方法:将40只雄性SD大鼠,采用随机数字法分为A空白组、B模型组、C双丹明目组、D阳性对照组4组,每组10只20眼。将B、C、D三组实验大鼠采用STZ 50mg/kg的剂量一次性大鼠尾静脉注射法建立糖尿病视网膜病变大鼠模型,造模后1wk开始连续灌胃用药,灌胃后4wk,处死动物,免疫组化法检测视网膜组织中VEGF-a、VEGF-b、VEGF-c的表达。结果:成模后用药第4wk,模型组、双丹明目组、阳性对照组视网膜中VEGF-a、VEGF-b、VEGF-c蛋白表达平均灰度值均低于正常组,平均光密度均高于正常组,其中模型组与正常组比较,差异均具有统计学意义(P<0. 01);双丹明目组、阳性对照组VEGF-a、VEGF-b、VEGF-c表达的平均灰度值均高于模型组(P<0. 05),平均光密度值均低于模型组(P<0. 01)。结论:双丹明目胶囊能明显降低VEGF家族中VEGF-a、VEGF-b、VEGF-c在糖尿病模型大鼠视网膜中的表达,对其视网膜有一定的保护作用。AIM： To study the effects of Shuangdan Mingmucapsule on the expressions of vascular endothelialgrowth factor - a （ VEGF - a）, VEGF - b, VEGF - c in theretina of a diabetic rat model. METHODS： Forty male SD rats were divided intoGroup A （ normal group）, Group B （model group）,Group C （ Shuangdan Mingmu group） and Group D（positive control group） 10 rats （20 eyes） in each group.A rat model of diabetic retinopathy was established byone- time tail vein injection with STZ （50mg/ kg）. Aftermodeling for 1wk, the rats were given medicine bygavage. After gavage for 4wk rats were sacrificed, andthe expressions of VEGF - a, VEGF - b, VEGF - c in theretina tissues were detected by immunohistochemicalmethod. RESULTS： After gavage for 4wk the average grayvalues of VEGF- a, VEGF- b and VEGF- c protein in the retina of model group, Shuangdan Mingmu group andpositive control group were lower than those of thenormal group, and the average optical density werehigher than those of the normal group. There was asignificant difference between the model group and thenormal group （ P 〈 0. 01 ）. The average gray values ofVEGF-a, VEGF-b and VEGF-c expression in ShuangdanMingmu group and positive control group were higherthan those in the model group （P〈0. 05） and the averageoptical density value were lower than those in the modelgroup. （P〈0. 01）. CONCLUSION： Shuangdan Mingmu capsule couldsignificantly reduce the expressions of VEGF-a, VEGF-b,VEGF-c in the retina and had a certain protective effect on the retina of rats in the diabetic retinopathy model.

关 键 词：双丹明目胶囊 糖尿病大鼠模型 VEGF-A VEGF-B VEGF-C 

分 类 号：R587.2[医药卫生—内分泌]