明胶/姜黄素纳米纤维电纺膜皮下环境构建组织工程软骨的可行性研究  

The Construction of Tissue Engineered Cartilage by Using Gelatin/Curcumin Nanofibres Membranes in Subcutaneous Model

作　　者：KANG BO KYOUNG 陈维明 周广东 曹德君[1] KANG Bokyoung;CHEN Weiming;ZHOU Guangdong;CAO Dejun(Department of Plastic and Reconstructive Surgery,Shanghai Ninth People＇s Hospital,Shanghai Jiaotong University School of Medicine,Shanghai 200011;Shanghai Key Laboratory of Tissue Engineering,Shanghai 200011;National Tissue Engineering Center of China,Shanghai 200241,China.)

机构地区：[1]上海交通大学医学院附属第九人民医院整复外科,上海市200011 [2]上海组织工程研究重点实验室,上海市200011 [3]组织工程研究国家工程中心,上海市200241

出　　处：《组织工程与重建外科杂志》2018年第5期245-249,共5页Journal of Tissue Engineering and Reconstructive Surgery

摘　　要：目的探讨以明胶/姜黄素电纺材料为支架,在动物皮下构建组织工程化软骨的可行性。方法取兔耳软骨获取软骨细胞作为种子细胞,体外培养扩增,并以明胶电纺膜(对照组)和明胶/姜黄素电纺膜(实验组)为支架构建软骨。将支架材料植入BALB/c裸鼠背部皮下后行大体观察;对支架材料及细胞-支架复合物行电镜观察;对细胞-支架复合物进行细胞增殖率检测;另将体外培养1周的细胞-支架复合物植入BALB/c裸鼠背部皮下,3周、12周后行组织学检测,比较不同时间点的软骨形成情况。结果大体观察显示,相比对照组,实验组材料周围组织血管化受到明显抑制;SEM结果显示,两组材料中细胞均黏附良好;细胞增殖率检测结果显示,姜黄素对细胞增殖无显著影响;组织学观察显示,3周、12周时,两组细胞-支架复合物均有软骨陷窝形成;但相比对照组,实验组材料可见明显吸收,软骨基质形成较厚,均质性更佳。结论明胶/姜黄素材料可作为支架在裸鼠皮下构建组织工程化软骨,具有潜在的应用前景。Objective To explore the feasibility of constructing tissue engineered cartilage by using gelatin/curcumin nanofibres membranes as scaffold in subcutaneous model. Methods The seed cells were obtained from rabbit ear cartilage. After in vitro culture and expansion, cartilage was constructed with gelatin nanofibers membrane （control group） and gelatin/curcumin nanofibers membrane （experimental group） as scaffolds. Gross observation was performed after the material was implanted into the back of the BALB/c nude mice; SEM observation of the material and cell-material complex were all performed; The cell proliferation rate of the cell-material complex was measured; The cell-material complex cultured in vitro for 1 week was implanted into the back of BALB/c nude mice. Histological examination was performed 3 and 12 weeks after implantation, and cartilage formation at different time points was compared. Results Gross observation showed that compared with the control group, the vascularization of the tissue around in the experimental group was significantly inhibited; The SEM observation showed that the chondrocyts in the two groups adhered well; Growth curve of the chondrocyts showed that curcumin had no significant effect on cell growth; Histological observation showed that the cell-material complex of both groups had cartilage lacuna formation after implanted for 3 and 12 weeks, but compared with the control group, the material in experimental group showed obvious absorption, and the cartilage matrix was thicker and more homogenization. Conclusion Gelatin/curcumin membranes could be successfully used as scaffold in the construction of tissue engineering cartilage in subcutaneous animal model. It is a kind of scaffold material with potential application prospect.

关 键 词：组织工程化软骨 纳米纤维电纺膜 姜黄素 抗血管化 

分 类 号：R318.08[医药卫生—生物医学工程]