护场理论指导下的如意金黄散加减酊剂箍围法对皮肤脓肿大鼠组织CD68、MPO表达的影响  

Influence of surrounding-hoop method of Modified Ruyi jinhuangsan Tincture Tincture,under guidance of huchang theory,on expressions of CD68 and myeloperoxidas in rats with skin abscess

作　　者：徐强[1] 刘婷婷[2] 王婉莹[2] 朱朝军[1] 张朝晖[1] Xu Qiang;Liu Tingting;Wang Wanying;Zhu Chaojun;Zhang Zhaohui(Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300250,China;Tianjin University of Tradi-tional Chinese Medicine,Tianjin 300193,China)

机构地区：[1]天津中医药大学第二附属医院,天津300250 [2]天津中医药大学

出　　处：《北京中医药大学学报》2018年第9期771-780,共10页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(No.81273759)~~

摘　　要：目的观察护场理论指导下的箍围药如意金黄散加减酊剂外用对皮肤脓肿大鼠周围局部组织巨噬细胞标记物(CD68)、髓过氧化物酶(MPO)表达的影响。方法将50只大鼠随机分为空白组、预治疗组、治疗组、对照组、模型组,除空白组外,其余4组皮下注射金黄色葡萄球菌造成皮下脓肿模型,采用如意金黄散加减酊剂、莫匹罗星软膏及生理盐水等不同药物外敷治疗。分别于治疗8、13、18、22、25 d提取大鼠脓肿周围1、2、3 cm皮下组织制成切片,免疫组化染色,采用显微镜结合光密度检测法检测不同时期、不同位置大鼠组织CD68及MPO阳性表达,并分析其变化及影响。结果各组大鼠脓肿周围组织CD68及MPO累积光密度值在治疗期间均呈先升后降的趋势,且随着距离脓肿中心距离减小阳性表达亦减弱。如意金黄散加减治疗组在脓肿尚未形成期及脓肿形成后期对局部CD68及MPO的影响呈双面性,在不同阶段的不同范围内,脓肿未形成期应用如意金黄散加减酊剂的预治疗组CD68及MPO表达值不同程度地低于其他治疗组,差异具有统计学意义(P <0. 05或P <0. 01),而脓肿形成后采用如意金黄散加减酊剂的治疗组CD68及MPO表达值则不同程度高于其他治疗组,差异具有统计学意义(P <0. 05或P <0. 01)。结论早期应用如意金黄散加减酊剂治疗大鼠皮肤脓肿,能够在脓肿形成不同时期调节局部促炎性因子标记物CD68及MPO的释放,从而为护场形成提供有利条件。Objective To observe the influence of surrounding-hoop method of Modified Ruyi jinhuangsan Tincture Tincture,under guidance of huchang theory,on expressions of macrophage markers（ CD68） and myeloperoxidas（ MPO） in rats with skin abscess. Methods All rats（ n = 50） were randomly divided into blank group,pretreatment group,treatment group,control group and model group.Except of blank group,other 4 groups were injected with Staphylococcus aureus to establish model of skin abscess,and treated externally with, respectively, Modified Ruyi jinhuangsan Tincture Tincture,Mupirocin Ointment and physiological saline. The samples of subcutaneous tissue around abscess for 1 cm,2 cm and 3 cm were collected for making into slices after treatment for 8 d,13 d,18 d,22 d and 25 d,and were given immunohistochemistry staining. The expressions,changes and influence of CD68 and MPO were detected and analyzed by using microscopy and integrated optical density（ IOD） detection.Results IOD values of CD68 and MPO showed a trend of firstly ascending and then descending during the treatment,and decreased with the increase of distance from abscess center and their positive expressions weakened also. The influence of Modified Ruyi jinhuangsan Tincture Tincture was two-sided on local abscess during abscess non-formation stage and late stage of abscess formation. The expressions of CD68 and MPO were lower in pretreatment group in varying degrees than those in other groups（ P 〈0. 05 or P 〈0. 01） at different stages and distances during abscess non-formation stage. The expressions of CD68 and MPO were higher in treatment group in varying degrees than those in other groups（ P 〈0. 05 or P 〈0. 01） after abscess formation. Conclusion The early administration of Modified Ruyi jinhuangsan Tincture Tincture for treatment skin abscess in rats can regulate the releases of CD68 and MPO,local proinflammatory cytokines,at different stages of abscess formation,and provide a favorable condition for huchang formation.

关 键 词：护场理论 箍围法 脓肿 巨噬细胞标记物 髓过氧化物酶 大鼠 

分 类 号：R285.5[医药卫生—中药学]