长三角地区设施作物上灰霉病菌的群体遗传结构分析  

Genetic structure of populations of the Botrytis cinerea in protected crops from Yangtze River Delta region

作　　者：李伟[1] 卢晓雪 张爱香[1] 周鑫 魏利辉[1] 陈怀谷[1] LI Wei1, LU Xiao-xue1, ZHANG Ai-xiang1 , ZHOU Xin2 , WEI Li-hui1 , CHEN Huai-gu1.(1 Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; 2Jiangsu Huai＇an District Shanghe Town Agro-Tech Extension and Service Center, Huai＇an 223239,China)

机构地区：[1]江苏省农业科学院植物保护研究所,南京210014 [2]江苏省淮安市淮安区上河镇农业技术推广服务中心,淮安223239

出　　处：《植物病理学报》2018年第5期624-631,共8页Acta Phytopathologica Sinica

基　　金：国家公益性行业(农业)科研专项(201303025);特色蔬菜产业技术体系建设项目(CARS-24-C-01)

摘　　要：长三角地区是我国设施作物生产水平和产量最高的地区之一,灰霉病在该地区发生普遍,严重影响作物的品质和产量。本研究从长三角地区(江苏盐城、南通、淮安、苏州、扬州、镇江,上海嘉定,浙江嘉兴、宁波)种植番茄、草莓等的温室大棚中采集了灰霉病菌195株,根据采集地点和寄主植物种类将这些菌株划分为16个群体,利用9对SSR引物对这些菌株进行了群体遗传结构分析。结果表明长三角地区灰霉病菌遗传多样性丰富,所分析的灰霉病菌的亲缘关系与采集地点的相关性不紧密,而与寄主植物种类有一定关系。进一步对27个大棚的菌株群体进行了遗传分析,在寄主植物相同的情况下,通常同一地点相邻大棚的菌株群体亲缘关系更近。遗传变异分子方差分析(AMOVA)发现灰霉病菌群体的变异主要来源于群体内部,但群体间的遗传变异比率非常高,表明群体间分化较大,温室大棚的存在对菌株的进化起到了明显的隔离作用。本研究结果为进一步分析温室大棚的使用对灰霉病菌遗传进化及病害流行的影响提供了基础。The Yangtze River Delta region is one of the most productive areas of protected crops in China. Gray mold disease caused by Botrytis cinerea is common in this region and seriously affects crop quality and yield. In this study, 195 isolates of B. cinerea were collected from greenhouse plantings such as tomato, strawberry and so on in the Yangtze River Delta region （Yancheng, Nantong, Hual＇an, Suzhou, Yangzhou and Zhenjiang city in Jiangsu Province; Jiading District in Shanghai; Jiaxing and Ningbo city in Zhejiang Province）. Sixteen popula- tions were divided according to the host plant and geographical position, and 9 microsatellite markers were used for population genetic structure analysis. The results showed that the genetic diversity of B. cinerea in the Yang- tze River Delta region was abundant. The genetic relationship of B. cinerea analyzed in this study was more relevant to the host plant species, but not the collection site. Further genetic analysis of the populations from 27 greenhouses indicated that the populations with same host plant from neighbor greenhouses usually have closer genetic relationship. Based on the analysis of molecular variance （ AMOVA）, most of the genetic variation of B. cinerea came from inside of the populations, but the genetic variation rate among the populations was very high. The results indicated that the differentiation among the populations was large, and a very low level of inoculum exchange occurred among greenhouses. This study will help us to further understand the impact of the greenhouse on the genetic evolution and the epidemic of B. cinerea, and provides a theoretical basis for making effective strategies to control gray mold on protected crops.

关 键 词：灰葡萄孢 设施作物 群体遗传 病害流行 

分 类 号：S432.44[农业科学—植物病理学]