小麦条锈菌转主寄主小檗的离体叶片接种培养鉴定  

Detached-leaf inoculation method for identifying barberry serving as an alternate host for Puccinia striiformis West. f. sp. tritici Erikss. et Henn.

作　　者：郑丹[1] 左淑霞[1] 杜志敏 赵杰[1] 康振生[1] ZHENG Dan, ZUO Shu-xia, DU Zhi-min, ZHAO Jie , KANG Zhen-sheng(Key State Laboratory of Stress Biology in Arid Areas, College of Plant Protection, Northwest A＆F University, Yangling 712100, China)

机构地区：[1]西北农林科技大学植物保护学院旱区作物逆境生物学国家重点实验室,杨凌712100

出　　处：《植物病理学报》2018年第5期707-710,共4页Acta Phytopathologica Sinica

基　　金：国家重点基础研究发展计划项目(2013CB127700);陕西省自然科学基金(2017JM3006);高等学校学科创新引智计划(B07049)

摘　　要：近年，小檗（Berberis）被鉴定是小麦条锈菌的转主寄主，从而发现了小麦条锈菌存在有性生殖，使得研究条锈菌与转主寄主小檗相关的生物学、遗传学成为可能。小麦条锈菌（Puccinia striiformis West．f．sp．tritici Erikss．et Henn．）是活体营养的专性寄生物，只能在活体上侵染繁殖。Puccinia striiformis West. f. sp. tritici Erikss. et Henn. （Pst）, is a heteroecious and obligate rust fungus,causing wheat stripe rust in the world. The rust completes sexual cycle on Berberis （mainly） and Mahonia serving as alternate hosts. However, propagation of Berberis plants from seeds to young seedlings is time consuming, hindering rapid identification of Berberis as alternate host for Pst. To know whether Pst completes sexual cycles, including pycnial stage and aecial stage, on Berberis in vitro, we tested each of two stages of sexual cycle Pst on the detached leaves of Berberis （B. shensiana Ahrendt） after the Berberis leaves were inoculated using basidiospores produced from germinated teliospores of Pst race CYR32 in a Petri dish. During cultivation of detached leaves in vitro since inoculation, only water-soaked cotton wrapped around leaf stalk of the leaves. The results showed that Pst performed pycnial and aecial stages on detached leaves of B. shensiana at 9 and 16 d after inoculation in vitro, similar to that of living Berberis plants. The resultant aeciospores were inoculated on wheat cultivar Mingxian 169 and produced a great deal of uredia and urediospores on wheat leaves at 15 d post inoculation, indicating that aeciospores produced on detached leaves of Berberis were vigorous. In vitro, duration of detached leaves of B. shensiana with supply of only water reached up to 35 days, much longer than maximum time length of sexual cycle of Pst completing on living Berberis plants. The present study demonstrated that in vitro test of Berberis susceptibility against Pst using detached young leaves of Berberis was effective and feasible, facilitating testing study of identification of Berberis to serveas alternate host for Pst or genetic study on Pst using Berberis.

关 键 词：小麦条锈菌 转主寄主 接种培养 离体叶片 小檗 鉴定 有性生殖 生物学 

分 类 号：S432.1[农业科学—植物病理学]