新型国产重组甘精胰岛素对1型糖尿病模型大鼠的降糖作用研究  

Study on hypoglycemic effect of novel domestic recombinant insulin glargine injection on diabetic model rats

作　　者：魏滋鸿 李薇[1] 武卫党[1] 李亚卓[1] 曾勇[1] 高晶[1] 司端运[1] 朱虹 路江杰 伊秀林[1] WEI Zihong1, LI Wei1, WU Weidang1, LI Yazhuo1, ZENG Yong1, GAO Jing1, SI Duanyun1, ZHU Hong2, LU Jiangjie2, YI Xiulin1(1. State Key Laboratory of Drug Release Technology and Pharmacokinetics, Tianjin Institute of Pharmaceutical Research New Drug Evaluation Research Co. Ltd,, Tianjin 300301, China 2. Tianmai Biological Technology Development Co., Ltd., Hefei 230601, China)

机构地区：[1]天津药物研究院新药评价有限公司,释药技术与药代动力学国家重点实验室,天津300301 [2]合肥天麦生物科技发展有限公司,安徽合肥230601

出　　处：《药物评价研究》2018年第8期1446-1449,共4页Drug Evaluation Research

摘　　要：目的探讨新型国产重组甘精胰岛素注射液对1型糖尿病模型大鼠的降糖作用。方法选择健康SD大鼠和链脲佐菌素诱导的1型糖尿病大鼠,甘精胰岛素注射液(来得时)为阳性对照药,健康SD大鼠单次sc 2、4 IU/kg剂量的重组甘精胰岛素注射液,大鼠尾静脉采血,分别测定0 h(给药前)及给药后1、2、4、6、8 h空腹血糖;1型糖尿病模型大鼠连续5d sc 4、8 IU/kg剂量的重组甘精胰岛素注射液,每天分别测定给药前(0 h)和给药后(4 h)空腹血糖,给药第1、5天测定给药后1、2、4、6、8 h空腹血糖。结果健康大鼠sc重组甘精胰岛素注射液2、4 IU/kg后,血糖值在2 h下降到最低,与对照组比较差异显著(P<0.01);模型大鼠连续5 d sc 4、8 IU/kg重组甘精胰岛素注射液,血糖于给药后4~6 h下降至最低点,与模型组比较差异显著(P<0.01);每天给药4 h后血糖均呈平稳下降趋势,与模型组比较差异显著(P<0.01),8 IU/kg血糖值与对照组接近。结论国产重组甘精胰岛素注射液对1型糖尿病模型大鼠有较好的降糖效果,与同剂量阳性药降糖效果相当。Objective To explore the hypoglycemic effect of novel domestic recombinant insulin glargine injection on type 1 diabetic model rats. Methods Healthy SD rats and type I diabetic model rats induced by streptozotocin were used as model animals. The insulin glargine injection（Lantus） were used as the positive control drug. Healthy SD rats were sc injected with recombinant insulin glargine injection at two doses of 2 and 4 IU/kg for one time. Blood samples were taken from the tail vein of rats, the blood glucose values of 0 h and 1, 2, 4, 6, and 8 h were determined after administration. Type 1 diabetic model rats were sc injected with recombinant insulin glargine injection at two doses of 4 and 8 IU/kg for 5 d. Fasting blood glucose was measured daily before and after administration（4 h）. Fasting blood glucose was measured at 1, 2, 4, 6, and 8 h after administration for days 1 and 5. Results After injected with 2 and 4 IU/kg, the blood glucose level was the lowest level in 2 h, which was significantly different compared with control group（P〈0.01）. After sc injection of recombinant insulin glargine injection at two dose of 4 and 8 IU/kg in the model rats, the blood sugar decreased to the lowest point after 4 — 6 h, which was significantly different compared with model group（P〈0.01）, and the blood glucose decreased steadily 4 h after administration per day, and was significantly different from the model group（P〈0.01）, and the blood sugar of 8 IU/kg group was close to control group. Conclusion The domestic recombinant insulin glargine injection have good effect on hypoglycemic in rats with type 1 diabetes.

关 键 词：1型糖尿病 重组甘精胰岛素注射液 链脲佐菌素 血糖 

分 类 号：R965.1[医药卫生—药理学]