广西特色壮药金边蚂蟥抗痛风活性成分研究  

Study on Active Ingredients of Anti-gout in Poecilobdella Manillensis of Guangxi Featured Zhuang Medicine

作　　者：刘喜华[1] 黄思诗 黄敏琪[1] 熊万娜[1] 周维海 甄汉深[3] 刘安韬[1] Liu Xihua;Huang Sishi;Huang Minqi;Xiong Wanna;Zhou Weihai;Zhen Hanshen;Liu Antao(Guangxi Medical College,Nanning 530021,China;Nanning Jinhai Keyken Biopharmtechnology Co.Ltd.,Nanning 530001,China;Guangxi University of Chinese Medicine,Nanning 530001,China)

机构地区：[1]广西卫生职业技术学院,南宁530023 [2]南宁市金海科康生物医药科技有限公司,南宁530001 [3]广西中医药大学,南宁530001

出　　处：《世界科学技术-中医药现代化》2018年第6期941-946,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：广西科技厅广西自然科学基金青年项目(2015GXNSFBA139150):广西特色壮药金边蚂蟥抗高尿酸血症物质基础研究,负责人:刘喜华;广西教育厅广西高校中青年教师基础能力提升项目(2017KY1228):广西特色壮药金边蚂蟥抗高尿酸血症物质优选研究,负责人:刘喜华;广西科技厅广西科学研究与技术开发重大专项计划(桂科重:1355001-5-9):广西菲牛蛭新品种选育及室内无土立体养殖技术研发,负责人:农韦健.

摘　　要：目的:研究金边蚂蟥中具有抗痛风作用的活性成分。方法:采用次黄嘌呤复制小鼠高尿酸血症模型,二甲苯诱导小鼠耳郭肿胀模型,热板法、扭体法筛选金边蚂蟥的活性部位,筛选金边蚂蟥活性成分,观察金边蚂蟥抗抗痛风作用的物质基础。结果:金边蚂蟥水溶性部位是其抗痛风的活性部位,可降低次黄嘌呤诱导的高尿酸血症小鼠的血清尿酸水平,可抑制二甲苯诱导的小鼠耳郭肿胀,可减少醋酸诱导的小鼠扭体反应和增加小鼠热板痛阈;水溶性部位中水蛭素是主要活性成分。金边蚂蟥活性成分(水蛭素)0.8 g/kg和0.4 g/kg及金边蚂蟥续滤粉2.0 g/kg可显著降低血清尿酸水平,血清尿酸水平由模型组的232.73±50.93 umol/L分别下降到140.70±25.97 umol/L、149.07±39.28 umol/L、176.45±44.33 umol/L(P <0.01);金边蚂蟥活性成分(水蛭素)0.8 g/kg、0.4 g/kg和0.2 g/kg及金边蚂蟥续滤粉2.0 g/kg可明显抑制二甲苯引起小鼠耳郭肿胀,肿胀度由22.80±2.86 mg分别抑制到20.10±2.18 mg、19.80±2.57 mg、20.10±1.66 mg、20.85±1.60 mg(P <0.05);金边蚂蟥活性成分(水蛭素)0.8 g/kg,可明显降低醋酸引起的小鼠扭体次数,次数由22.80±2.86次降到20.10±2.18次(P <0.05)。结论:金边蚂蟥抗痛风的活性部位是水溶性部分,其中水蛭素是其主要活性成分。Objective： To study the anti-gout effect of active ingredients in Poecilobdella manillensis. Methods： Hypoxanthine was used to replicate mouse model of hyperuricemia, and xylene was used to induce mouse auricle swelling model. The hot plate method and writhing method were used to screen the active site of Poecilobdella manillensis, and then the active ingredients were screened. The material basis of anti-gout effect of Poecilobdella manillensis was observed. Results： The water-soluble fraction of Poecilobdella manillensis was the active site against gout, which could reduce the level of serum uric acid in hypoxanthine-induced hyperuricemic mice and inhibit xylene-induced auricular swelling in mice, deduce acetic acid-induced writhing reaction in mice and increase the hot plate pain threshold in mice; Hirudin was the main active ingredient in water-soluble parts. Poecilobdella manillensis active ingredient 0.8 g/kg and 0.4 g/kg and Poecilobdella manillensis residue 2.0 g/kg could significantly reduce serum uric acid levels. The serum uric acid levels decreased from 232.73 ±50.93 umol/L in model group to 140.70 ±25.97 umol/L, 149.07 ±39.28 umol/L, 176.45 ±44.33 umol/L, respectively （P 〈 0.01）. Poecilobdella manillensis active ingredients 0.8 g/kg, 0.4 g/kg and 0.2 g/kg and Poecilobdella manillensis residue 2.0 g/kg could significantly inhibit xylene-induced ear auricle swelling in mice. The swelling degree was inhibited from 22.80 ±2.86 mg to 20.10 ±2.18 mg, 19.80 ±2.57 mg, 20.10 ±1.66 mg and 20.85 ±1.60 mg respectively （P 〈 0.05）. Poecilobdella manillensis 0.8 g/kg active ingredient could significantly reduce the number of writhing mice caused by acetic acid. The number of times was reduced from 22.80 ±2.86 times to 20.10 ±2.18 times （P 〈 0.05）. Conclusion： Poecilobdella manillensis anti-gout activity is in water-soluble parts, and Hirudin is the main active ingredient.

关 键 词：金边蚂蟥 水蛭素 高尿酸 炎症 镇痛 

分 类 号：R29[医药卫生—民族医学]