不同电针对快速老龄化痴呆小鼠行为学和海马星形胶质细胞的影响  

The Effects of Different Electro-acupuncture Intervention on Learning and Memory Abilities and Expression of Hippocampal Astrocytes in Rapid Aging Mouse Model of Senia Dementia

作　　者：邵淑君 唐银杉[2] 曹瑾[1] 许安萍[1] 田朝阳 郭郁[1] 向杜炼 李志刚[1] 邬继红[1] Shao Shujun;Tang Yinshan;Cao Jin;Xu Anping;Tian Zhaoyang;Guo Yu;Xiang Dulian;Li Zhigang;Wu Jihong(School of Acu-moxibustion and Tuina,Beijing University of Chinese Medicine,Beijing 10029,China;Second Affiliated Hospital of Zhejiang University School of Traditional Chinese Medicine Rehabilitation,Hangzhou 310000,China)

机构地区：[1]北京中医药大学针灸推拿学院,北京100029 [2]浙江大学医学院附属第二医院中医康复科,杭州310000

出　　处：《世界科学技术-中医药现代化》2018年第6期953-959,共7页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金委青年基金项目(81603678):督脉电针治疗AD双转基因小鼠不同脑区神经元凋亡作用机制的研究;负责人:唐银杉;国家自然科学基金委青年基金项目(81503654):逆针灸对老年性痴呆小鼠"抗衰老基因"Klotho的调控;负责人:许安萍

摘　　要：目的:探讨音乐电针和脉冲电针对快速老龄化(SAMP8)小鼠行为学和海马星形胶质细胞的影响,对比观察其抗痴呆的作用机制。方法:8月龄雄性SAMP8小鼠共30只,随机分为模型组,音乐电针组,脉冲电针组(n=10),以同源的正常老化(SAMR1)小鼠为空白组(n=10),选取"百会"、"印堂"、"人中",每天针刺干预1次,共15天。采用Morris水迷宫观察各组小鼠学习记忆能力变化,运用免疫组化法观察海马GFAP的表达,Western blot检测海马GFAP的蛋白水平。结果:Morris水迷宫提示:与空白组相比,模型组的逃避潜伏期延长(P <0.01),空间探索实验游泳距离增长(P <0.01);与模型组相比,音乐电针组和脉冲电针组逃避潜伏期缩短(P <0.01,P <0.05),游泳路程缩短(均P <0.05)。免疫组化结果:与空白组相比,模型组海马CA1区GFAP平均光密度明显增多(P <0.01);与模型组相比,音乐电针组和脉冲电针组海马CA1区GFAP平均光密度均明显降低(均P <0.01),且音乐电针组GFAP平均光密度低于脉冲电针组(P <0.05)。Western blot检测:与空白组相比,模型组海马GFAP蛋白表达明显增多(P <0.01);与模型组相比,音乐电针组和脉冲电针组GFAP蛋白表达有明显变浅和下降趋势(均P <0.05);音乐电针组GFAP蛋白表达明显低于脉冲电针组(P <0.05)。结论:音乐电针和脉冲电针均可改善SAMP8小鼠学习记忆能力,降低GFAP的表达,可能与其减轻神经炎性反应、改善凋亡,最终达到保护神经元的机制有关,且音乐电针有优于脉冲电针的趋势。Objective： To observe the effects of music electro-acupuncture and pulsed electro-acupuncture on locomotor avtivity and hippocampal astrocytes in fast-aging （SAMP8） mice, and to compare the anti-dementia mechanism. Methods： Thirty 8-month-old male SAMP8 mice were randomly divided into model group（group M）, music electro- acupuncture group（group MA）, pulsed electro-acupuncture group（group EA）（n=lO）, with homologous normal aging SAMR1 mice as control group（group C）（n=10）. Acupuncture stimulation was applied to ＂Baihui＂ （GV20）, ＂Yintang＂ （GV29） and ＂Renzhong＂（DU26） for 20 rain per day for 15 days. The Morris water maze was used to assess learning- memorize ability. Immunohistochemical DAB staining was used to observe GFAP exptession in hippocampus. Detect GFAP protein levels by Western blot in hippocampus. Results： The Morris water maze test showed ： Compared with group C, the escape latency were increased （P 〈 0.01）, swimming distance were increased in space exploration in group M （P 〈 0.01）. Compared with group M, the escape latency were reduced in group MA and EA （P 〈 0.01, P 〈 0.05） and the swimming distance were shortened （all P 〈 0.05）. The immunohistochemical DAB staining showed： Compared with group C, the average optical density of GFAP in the hippocampal CA I area of group M increased significantly （P 〈 0.01）; the optical density of GFAP in CA1 area of hippocampus in both groups MA and EA was significantly decreased （all P 〈 0.01）, while the optical density of GFAP in MA group was lower than that of EA group （P 〈 0.05）. Western blot test showed： Compared with group C, the expression of GFAP protein obviously increased （P 〈 0.01）. Compared with group M, the expression of GFAP protein obviously decreased and had a downregulation tendency in group MA and EA （all P 〈 0.05）, while the expression of GFAP protein decreased in MA group compare with EA group （P 〈 0.05）. Conclusion： Both mu

关 键 词：阿尔茨海默病 音乐电针 脉冲电针 胶质纤维酸性蛋白 星形胶质细胞 

分 类 号：R245.31[医药卫生—针灸推拿学]