HSV1和HSV2对呼吸道及阴道上皮细胞的感染及其诱导的细胞内固有免疫应答信号分子表达特征分析  

Expression characteristics of signaling molecules associated with innate immune response induced by HSV1 and HSV2 in respiratory and vaginal epithelial cells

作　　者：廖芸[1] 廉亚茹 李琦涵[1] 范胜涛[1] 柳蕾 王建斌[1] 冯敏[1] 刘镓玮 张莹[1] Liao Yun;Lian Yam;Li Qihan;Fan Shengtao;Liu Lei;Wang Jianbin;Feng Min;Liu Jiawei;Zhang Ying Yunnan(Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases,Institute of Medical Biology,Chinese Academy of Medical Sciences and Peking Union Medical College,Kunming 650118,China（Liao Yun,Lian Yaru,Li Qihan,Fan Shengtao,Liu Lei,Wang Jianbin,Feng Min,Liu Jiawei,Zhang Ying;Jiangsu Convac Biotechnology Co.,Ltd,Taizhou 225300,China（Lian Yaru)

机构地区：[1]中国医学科学院北京协和医学院医学生物学研究所云南省传染病疫苗重点实验室,昆明650118 [2]江苏康淮生物技术股份有限公司,泰州225300

出　　处：《中华微生物学和免疫学杂志》2018年第9期641-651,共11页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金（31670173）;中国医学科学院医学与健康科技创新工程项目（2016-12M-1-019）;云南省科技计划重大专项（2017ZF020,2017ZF006）

摘　　要：目的 探讨1型和2型单纯疱疹病毒（HSV1和HSV2）感染对诱导呼吸道及生殖器上皮细胞中固有免疫信号分子表达的影响,以期初步了解单纯疱疹病毒原发性感染部位——上皮组织中的感染特征以及病理学特性.方法 利用光学显微镜及扫描电镜观察病毒感染人胚肺细胞KMB17及人阴道上皮VK2细胞后,细胞形态及内部结构的变化;采用蚀斑法和微孔细胞病变法,并结合实时定量PCR,检测病毒在两种细胞中的增殖情况;使用实时定量PCR方法分析病毒感染两种细胞后,诱导的固有免疫信号分子的表达情况.结果 HSV1和HSV2均能够感染KMB17及VK2细胞,并在12 h后出现明显的细胞病变及内部微结构破坏损伤的现象.两种病毒在两种细胞单层上均能够形成形态各异的蚀斑,但HSV2在KMB17细胞和VK2细胞上增殖速度慢于HSV1.两种病毒感染两种细胞后,诱导的固有免疫相关的信号分子表达特征有相应的差别.结论 HSV1和HSV2均能以动力学增殖模式感染呼吸道及阴道上皮细胞,两种病毒在两种细胞上的增殖特性稍有区别,但其诱导固有免疫应答信号分子的表达特征有显著差异.Objective To investigate the influences of herpes simplex virus 1 and 2 （ HSV1 and HSV2） infection on the expression of signaling molecules associated with innate immune response in respira-tory and vaginal epithelial cells for bettering understanding of HSV infection and pathological characteristics in the primary infection site, namely mucosal epithelial tissues. Methods KMB17 and VK2 cells were in-fected with HSV. Changes in cell morphology and inner structure after HSV infection were observed under optical microscope and scanning electron microscope, respectively. Viral proliferation in KMB17 and VK2 cells was detected by plaque assay, microcytopathic assay and real-time quantitative PCR. Expression of sig-naling molecules associated with innate immune response in virus-infected KMB17 and VK2 cells were ana-lyzed by real-time quantitative PCR. Results Both HSV1 and HSV2 could infect KMB17 and VK2 cells, and cause damage to cell morphology and inner structure after 12 hours. Both of the two viruses formed simi-lar plaque on the single layer of KMB17 and VK2 cells, although HSV2 proliferated slower than HSV1. There were differences in the expression of signaling molecules associated with innate immune response in-duced by the two viruses in KMB17 and VK2 cells. Conclusion Both HSV1 and HSV2 could infect and proliferate in epithelial cells （ KMB17 and VK2 cells） . Although there were slight differences in viral prolif-eration between them, significant differences in the expression of signaling molecules associated with innate immune response induced by the two viruses were observed.

关 键 词：单纯疱疹病毒 上皮细胞 固有免疫应答信号分子 

分 类 号：R392[医药卫生—免疫学]