不同氢氧化铝佐剂和吸附方式对无细胞百白破-Sabin株脊髓灰质炎联合疫苗免疫效果的影响  

Effects of different concentrations of aluminum hydroxide adjuvant and adsorption methods on the immunogenicity of diphtheria-tetanus-acellular pertussis and inactivated poliovirus combined vaccine

作　　者：粱疆莉 顾琴[1] 高娜[1] 马艳[1] 姬秋彦[1] 史荔[1] 孙明波[1] 衡燮[1] Liang Jiangli;Gu Qin;Gao Na;Ma Yah;Ji Qiuyan;Shi Li;Sun Mingbo;Heng Xie(Institute of Medical Biology,Peking Union Medical College,Chinese Academy of Medical Sciences,Kunming 650118,China)

机构地区：[1]中国医学科学院北京协和医学院医学生物学研究所,昆明650118

出　　处：《中华微生物学和免疫学杂志》2018年第9期697-701,共5页Chinese Journal of Microbiology and Immunology

基　　金：国家科技重大专项课题（2015ZX09101031）;中国医学科学院重大协同创新项目“创新性疫苗研究”（2016-12M-1-019）

摘　　要：目的 研究不同氢氧化铝佐剂含量、两种吸附方式以及不同厂家氢氧化铝佐剂对无细胞百白破-Sabin株脊髓灰质炎联合疫苗（DTaP-sIPV）免疫效果的影响.方法 用0.42 mg/ml、0.47 mg/ml、0.52 mg/ml终浓度的铝吸附相同剂量的百白破5种抗原,用顺序吸附和分别吸附两种方式吸附抗原,使用进口和自制氢氧化铝,比较各抗原的吸附率、各抗原抗体水平以及百日咳、破伤风、白喉疫苗效价.结果 0.52 mg/ml铝含量组吸附率最高,0.42 mg/ml铝含量组吸附率最低;顺序吸附和分别吸附两种吸附方式没有明显差异;使用进口氢氧化铝佐剂和自制氢氧化铝佐剂组分抗体反应各有不同,差异无统计学意义.结论 0.52 mg/ml铝含量、自制氢氧化铝佐剂和分别吸附的方式适用于DTaP-sIPV疫苗的制备.Objective To investigate the effects of different concentrations and adsorption methods of aluminum hydroxide adjuvant produced by different manufacturers on the immunogenicity of the diphtheria-tetanus-acellular pertussis and inactivated poliovirus combined vaccine （ DTaP-sIPV） . Methods Five anti-gens of DTaP were adsorbed onto different concentrations （0. 42 mg/ml, 0. 47 mg/ml and 0. 52 mg/ml） of aluminum hydroxide from different manufacturers through sequential and separate adsorption. Adsorbability, anti-pertussis toxin （ PT）/filamentous hemagglutinin （ FHA）/pertactin （ PRN）/diphtheria toxoid （ DT）/tet-anus toxoid （ TT） antibodies and the potency of vaccines were detected. Results The adsorbability of alu-minum hydroxide adjuvant slightly decreased with the reduction of concentration. No significant difference in potency and antibody level was observed between sequential and separate adsorption. Moreover, no signifi-cant difference in antibody level was observed between vaccines prepared with aluminum hydroxide adjuvant produced by General Chemical Corp and our institute. Conclusion Aluminum hydroxide adjuvant produced by our institute at the concentration of 0. 52 mg/ml and separate adsorption method are suitable for prepara-tion of DTaP-sIPV.

关 键 词：DTaP—sIPV 吸附率 疫苗效价 抗百日咳毒素抗体 抗丝状血凝素抗体 抗百日 咳粘着素抗体 抗白喉类毒素抗体 抗破伤风类毒素抗体 

分 类 号：R392-33[医药卫生—免疫学]