基于GPER2/MAPK/STAT1轴研究少腹逐瘀汤治疗子宫内膜异位症痛经分子机制  

Molecular mechanism of Shaofu Zhuyu decoction in treating endometriosis dysmenorrhea based on GPER2/MAPK/STAT1 axis

作　　者：崔宇红[1] 吴晶[1] 蔡玮[2] 杜娟[1] 曹军民 吉秀家[1] 巩子汉 CUI Yu-hong,WU Jing,CAI Wei,DU Juan,CAO Jun-min,JI Xiu-jia,GONG Zi-han1(1.Gansu University of Chinese Medicine, Lanzhou 730000, China;2.Gansu Provincial Hospital, Lanzhou 730000, China)

机构地区：[1]甘肃中医药大学,甘肃兰州730000 [2]甘肃省人民医院,甘肃兰州730000

出　　处：《中国中药杂志》2018年第16期3362-3367,共6页China Journal of Chinese Materia Medica

基　　金：甘肃省自然科学基金项目(1508RJZA020);国家自然科学基金项目(81760879)

摘　　要：该研究基于GPER2／MAPK／sTAT1轴研究少腹逐瘀汤治疗子宫内膜异位症痛经分子机制。采用异位移植造EMT模型；HE染色观察各组大鼠灌药治疗后宫腔组织病理变化；EEISA实验检测大鼠宫腔组织中TNF-α，IL一6的含量变化；qPCR检测宫腔组织中神经激肽受体（NK1）、GPER mRNA表达；Western blot法检测官腔组织中MAPK，STAT1蛋白含量。结果显示，在病理水平上，与模型组相比，少腹逐瘀汤可显著改善异位宫腔组织炎症情况，导致异位宫腔组织中浸润的炎症细胞减少，抑制血管生成，使宫腔组织壁变薄，阻止其生长，这种抑制表现出一定的浓度依赖性。在分子水平上，少腹逐瘀汤降低宫腔组织中炎症相关因子TNF-α，IL-6含量，减少NK1，GPER2 mRNA的相对表达量，并导致宫腔组织中MAPK和STAT1蛋白表达降低。综上可知少腹逐瘀汤可有效抑制异位宫腔组织中GPER2 mRNA以及MAPK，STAT1蛋白的表达，降低TNF-α，IL-6相关炎症因子的含量以及疼痛介质NK1 mRNA的含量从而减轻子宫内膜异位症患者炎症疼痛。The aim of this paper is to study the molecular mechanism of Shaofu Zhuyu decoction in treating dysmenorrhea of endometriosis based on GPER2/MAPK/STAT1 axis. In this study, HE staining was used to observe the pathological changes of the rats in each group. The levels of TNF-α and IL-6 were detected by ELISA assay. The mRNA expressions of neurotransmitter receptor （NK1） and GPER were detected by qPCR. The protein contents of MAPK and STAT1 were detected by Western blot. According to the results, compared with the model group, Shaofu Zhuyu decoction could significantly improve the inflammation of the ectopic uterine cavity tissue, decrease the contents of TNF-a and IL-6 in the uterine cavity, the mRNA expressions of NK1 and GPER, and the protein expressions of MAPK and STAT1. In conclusion, Shaofu Zhuyu decoction could effectively inhibit the expressions of GPER2, MAPK and STAT1, decrease the levels of TNF-α, IL-6 and NK1 mRNA and relieve the inflammatory pain in patients with endometriosis.

关 键 词：少腹逐瘀汤 子宫内膜异位症 痛经 GPER2/MAPK/sTAT1轴 

分 类 号：R285.5[医药卫生—中药学]