HPLC同时测定吴茱萸水提物中的4个成分  

Simultaneous determination of four components in the water extract of Euodia rutaecarpa by HPLC

作　　者：张敏 李斐 董嘉皓 魏飞亭 刘超 袁金斌[2] ZHANG Min;LI Fei;DONG Jiahao;WEI Feiting;LIU Chao;YUAN Jinbin(Nanehang Key Laboratory of Quality Control and Safety Evaluation of Traditional Chinese Medicine,Nanchang Institute For Food and Drug Control Nanchang,Nanchang,Jiangxi,330015 P.R.China;Key Laboratory of Modern Preparation of Traditional Chinese Medicine,Ministry of Education,School of Pharmacy,Jiangxi University of Traditional Chinese Medicine,Nanchang,Jiangxi,330015 P.R.China)

机构地区：[1]南昌市食品药品检验所南昌市中药质控与安全性评价重点实验室,江西南昌330015 [2]江西中医药大学药学院现代中药制剂教育部重点实验室,江西南昌330004

出　　处：《华西药学杂志》2018年第5期523-525,共3页West China Journal of Pharmaceutical Sciences

基　　金：国家自然科学基金资助项目(批准号:81560648)

摘　　要：目的采用HPLC法同时测定吴茱萸水提物中绿原酸、芦丁、金丝桃苷、去氢吴茱萸碱的含量。方法采用C_（18）色谱柱（100 mm×4. 6 mm,3. 5μm）,流动相为乙腈-0. 1%磷酸水混合溶液,梯度洗脱,流速1. 0 mL·min（-1）,检测波长326 nm,进样量5μL,柱温30℃。结果绿原酸、芦丁、金丝桃苷、去氢吴茱萸碱的线性范围分别为25. 3～253、0. 501～50. 1、0. 252～25. 2、5. 03～503μg·mL（-1）（r2≥0. 9995）,平均加样回收率为100. 0%～100. 9%,RSDs均≤0. 8%。结论所用方法操作简便、结果准确,重复性和稳定性均较好,可作为吴茱萸药材质量控制的方法。OBJECTIVE To simultaneous determine the chlorogenic acid,rutin,hyperoside and dehydroevodiamine in the water extracts of Euodia rutaecarpa. by HPLC. METHODS Separation was performed on an C_（18） column（100 mm × 4. 6 mm,3. 5 μm）. The mobile phase consisted of acetonitrile-0. 1% phosphoric acid water with a gradient of 1. 0 mL·min（-1）. The detection wavelength was326 nm with the injection volume of 5 μL and the column temperature of 30 ℃. RESULTS The calibration curves of all four constituents showed good linearity（ r2≥0. 9995） in a relatively wide concentration ranges. The linear ranges of chlorogenic acid,rutin,hyperoside and dehydroevodiamine were 25. 3-253 μg·mL（-1）,0. 501-50. 1 μg·mL（-1）,0. 252-25. 2 μg·mL（-1） and 5. 03-503 μg·mL（-1）,respectively. The mean recoveries of the four components were 100. 0%-100. 9% with RSDs≤0. 8%. CONCLUSION The method is simple and accurate,and it has excellent repeatability and stability. It can be used as the quality control method for E.rutaecarpa.

关 键 词：吴茱萸 水提物 高效液相色谱法 同时测定 绿原酸 芦丁 金丝桃苷 去氢吴茱萸碱 质量控制 

分 类 号：R917[医药卫生—药物分析学]