FoxP3相关微RNA-210微RNA-155及微RNA-34a在系统性红斑狼疮患者外周血的表达及临床意义  

The expression and clinical significance of miR-210, miR-155 and miR-34a in peripheral blood of patients with systemic lupus erythematosus

作　　者：王友庆[1] 陈士芳[1] 梅珏[1] 许瑜佳[1] Wang Youqing;Chen Shifang;Mei Jue;Xu Yujia(Department of Rheumatology,Huzhou Central Hospital,Zhejiang 313000,China)

机构地区：[1]浙江省湖州市中心医院风湿免疫科,313000

出　　处：《中华风湿病学杂志》2018年第9期603-608,共6页Chinese Journal of Rheumatology

摘　　要：目的探讨微RNA（miR）-210、miR-155及miR-34a在SLE患者外周血的表达情况及临床意义。方法将126例SLE患者根据SLEDAI评分分为稳定组（35例）、轻度活动组（49例）和中重度活动组（42例），同时选取40名健康体检者作为对照组，收集患者临床资料，检测血常规、肝肾功能、免疫学指标，并评估狼疮活动指数；采用实时荧光定量（RT）-qPCR的方法检测患者外周血miR-210、miR-155及miR-34a的水平，并分析其与患者临床特征之间的关系；各组血清miR-210、miR-155、miR-34a的表达量比较采用单因素方差分析进行统计；相关性分析采用非参数Spearman相关分析；应用受试者工作特征曲线（ROC曲线）评价各指标对SLE的诊断价值。结果miR-210和miR-155的表达在对照组（0.015±0.010）；（0.071±0.034）、稳定组（0.017±0.012）和（0.150±0.101）之间差异无统计学意义，但轻度活动组（0.502±0.166）、（1.521±1.138）和中重度活动组（1.237±0.584）、（13.589±9.827）依次升高（F=124.321，70.065，P均〈0.05）；miR-34a的表达在对照组、稳定组、轻度活动组和中重度活动组的平均表达水平分别为（0.005±0.003）、（0.249±0.137）、（2.981±1.762）、（9.625±5.873），呈现依次升高的趋势（F=75.668，P〈0.05）；miR-210、miR-155、miR-34a在LN和非LN患者中的表达差异无统计学意义（P〉0.05）。miR-210、miR-155、miR-34a的表达与免疫球蛋白（Ig）G、IgA、IgM、ESR、C3、C4、ANA滴度、抗dsDNA抗体及SLEDAL评分均有明显的相关关系（P〈0.05），r值分别为：IgG（0.347；0.518；0.482），IgA（0.463；0.635；0.379），IgM（0.287；0.392；0.336），ESR（0.317；0.428；0.369），C3（0.243；0.429；0.381），C4（0.317；0.513；0.429），ANA滴度（0.462；0.594；0.527），抗dsDNA抗体（0.391；0.586；0.483），SLEDAI评分（-0.412；-0.558；-0.493）。在SLE患者中，无论是单用激素治疗或是�Objective To examine the expression of miR-210, miR-155 and miR-34a in the peripheral blood of patients with systemic lupus erythematosus （SLE） and to analyze their clinical significance.Methods One hundred and teenty-six SLE patients were divided into the stable group （n=35）, mild active group （n=49）, medium and severe active group （n=42） based on systemic lupus erythematosus disease activity index （SLEDAI） score. Meanwhile, 40 subjects for healthy check-up were selected as controls. The clinical data of patients were collected. Complete blood count, liver and kidney function and immunological indexes were tested in patients and the lupus activity index was assessed. The expression level of miR-210, miR-155 and miR-34a were determined by real-time polymerase chain reaction. Furthermore, the correlation between their levels and clinical parameters such as erythrocyte sedimentation rate （ESR）, C-reactive protein （CRP）, SLEDAI score, were analyzed. The expression levels of miR-210, miR-155 and miR-34a in each group were compared by one-way analysis of variance （ANOVA）. Correlation analysis was performed by non parametric Spearman test. The difference was statistically significant if P〈0.05. The diagnostic value of SLE was evaluated by the subjects characteristic （ROC） curve.Results The levels of miR-210 and miR-155 were no significantly differencet between the stable group （0.017±0.012）; （0.150±0.101） and the control group （0.015±0.010）; （0.071±0.034）, but they were increased in mild active group （0.502±0.166）; （1.521±1.138）, medium and severe active group（1.237±0.584）, （13.589±9.827） （F=124.321, 70.065, P〈0.05）. The average expression level of miR-34a in the control group, the stable group, the mild active group and the moderate and severe active group were （0.005±0.003）, （0.249±0.137）, （2.981±1.762） and （9.625±5.873） respectively, and showed a trend of increase in turn （F=75.688, P〈0.05）. The expression l

关 键 词：红斑狼疮 系统性 微RNAS 逆转录聚合酶链反应 

分 类 号：R593.241[医药卫生—内科学]