乙肝表面抗原和乙肝e抗原均阳性产妇乙肝病毒宫内感染情况及与Toll样受体4和干扰素-γ的相关性分析  

HBV intrauterine infection in HBs Ag and HBeAg positive women and its correlation with TLR4 and interferon-γ

作　　者：王菲菲[1] 马小星[1] WANG Fei-fei;MA Xiao-xing(Central Delivery Room,Yan＇an People＇s Hospital,Yan an Shaanxi 716000,P.R.China)

机构地区：[1]延安市人民医院中心产房,陕西延安716000

出　　处：《中国计划生育和妇产科》2018年第10期78-80,共3页Chinese Journal of Family Planning & Gynecotokology

摘　　要：目的分析探讨乙肝表面抗原(hepatitis B surface antigens,HBs Ag)和乙型肝炎e抗原(hepatitis B e antigens,HBe Ag)均阳性产妇乙型肝炎病毒(hepatitis B virus,HBV)宫内感染情况及与Toll样受体4 (Tolllike receptor 4,TLR 4)和干扰素-γ的相关性。方法回顾性分析延安市人民医院2015年1月至2017年2月收治的198例HBs Ag和HBe Ag均阳性产妇的临床资料,根据产妇是否出现HBV宫内感染将其分为甲乙两组,有HBV宫内感染的100例为甲组,无HBV宫内感染的98例为乙组。分别测定两组产妇分娩前的外周血HBV DNA含量和外周血干扰素-γ水平,分娩后脐带血中HBe Ag和HBs Ag水平,利用免疫组化测定两组产妇胎盘TLR 4和干扰素-γ等的表达情况。结果分娩前甲组产妇干扰素-γ水平[(212. 38±35. 49) pg/m L]低于乙组产妇[(1 279. 39±126. 38) pg/m L](P <0. 05);甲组产妇的HBV DNA水平[(6. 36±0. 36)×10~3copies/m L]高于乙组产妇[(0. 18±0. 03)×10~3copies/m L](P <0. 05)。甲组产妇分娩后脐带血中的HBe Ag和HBs Ag水平分别为(2. 23±0. 31) PEI U/m L和(3. 41±0. 32) ng/m L,均高于乙组产妇的(0. 32±0. 12) PEI U/m L和(0. 30±0. 09) ng/m L(P <0. 05)。甲组产妇分娩后的脐带血中TLR 4和相应m RNA以及干扰素-γ水平分别为(36. 49±6. 12) pg/m L、(0. 71±0. 13) pg/m L、(79. 17±10. 98) pg/m L,均低于乙组产妇的(49. 32±7. 18) pg/m L、(0. 97±0. 12) pg/m L、(114. 38±18. 92) pg/m L(P <0. 05)。结论干扰素-γ和TLR4在抑制HBs Ag和HBe Ag均阳性产妇HBV宫内感染中起重要作用,对于预防和控制HBV孕产妇宫内感染具有较高的临床价值。Objective To analyze hepatitis B surface antigens （HBsAg） and hepatitis B e antigens （HBeAg） positive for maternal hepatitis B virus （HBV） intrauterine infection and their correlation of TLR4 and interferon - γ （ INF - γ）. Methods Retrospectively analyzed the clinical data of 198 HBsAg and HBeAg - positive women admitted to Yan＇an People＇s Hospital from January 2015 to February 2017. According to whether the mother had HBV intrauterine infection,they were divided into two groups. 100 cases （ group A） were with HBV intrauterine infection, and 98 cases（ group B）were without HBV intrauterine infection. The peripheral blood HBV - DNA content and peripheral blood interferon - γlevels were measured before delivery. The levels of HBeAg and HBsAg in the cord blood of the two groups were measured. The immunological histochemistry was used to determine the maternal placental Toll - like expression of receptor 4 and interferon - γ, etc. Results The interferon - γlevel of the group A was （212. 38 ±35.49 ） pg/mL before delivery, which was significantly lower than that of the group B （ 1 279. 39 ± 126. 38） pg/mL （t =78. 282, P 〈0. 05）. The level of HBV DNA in group A was （6. 36 ±0. 36） × 10^3 copies/mL, which was significantly higher than that in group B （0. 18 ±0.03） × 10^3 copies/mL （t =236. 383, P 〈0. 05）. The levels of HBeAg and HBsAg in umbilical cord blood of group A were （2. 23 ±0. 31 ） PEI U/mL and （3.41 ±0. 32） ng/mL, respectively, which were significantly higher than those of group B （0. 32 ±0. 12） PEI U/mL and （0. 30 ± 0. 09） ng/mL （t = 64. 265, 93. 382, P 〈 0. 05）. The levels of TLR4, mRNA and INF - γ in umbilical cord blood of group A were （36. 49 ± 6. 12） pg/mL, （0. 71± 0. 13 ）pg/mL, （79. 17 ± 10. 98 ）mg/mL, respectively, which were significantly lower than those of group B （49. 32 ±7.18）pg/mL, （0. 97 ±0. 12） pg/mL, （ 114. 38 ±18. 92） pg/mL（P 〈0. 05）. Conclusion Interferon - γ and Tol

关 键 词：乙型肝炎病毒 宫内感染 干扰素 TOLL样受体4 相关性 

分 类 号：R714.7[医药卫生—妇产科学]