Profiling and identification of chemical components of Shenshao Tablet and its absorbed components in rats by comprehensive HPLC/DAD/ESI-MS^n analysis  

作　　者：MIAO Wen-Juan HU Ying JIA Li ZHANG Chun-Xia YANG Wen-Zhi ZHANG Ping GUO De-An 

机构地区：[1]Department of Pharmacy,Institute of Hematology and Blood Disease Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Tianjin 300020,China [2]Tianjin State Key Laboratory of Modern Chinese Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China [3]State Key Laboratory of Natural and Biomimetic Drugs,School of Pharmaceutical Sciences,Peking University,Beijing 100191,China

出　　处：《Chinese Journal of Natural Medicines》2018年第10期791-800,共10页中国天然药物（英文版）

基　　金：financially supported by Tianjin Municipal Education Commission Research Project(No.2017ZD07)

摘　　要：Shenshao Tablet(SST),prepared from Paeoniae Radix Alba(PRA)and total ginsenoside of Ginseng Stems and Leaves(GSL),is a traditional Chinese medicine(TCM)preparation prescribed to treat coronary heart disease.However,its chemical composition and the components that can migrate into blood potentially exerting the therapeutic effects have rarely been elucidated.We developed an HPLC/DAD/ESI-MS^n approach aiming to comprehensively profile and identify both the chemical components of SST and its absorbed ingredients(and metabolites)in rat plasma and urine.Chromatographic separation was performed on an Agilent Eclipse XDB C_(18) column using acetonitrile/0.1% formic acid as the mobile phase.MS detection was conducted in both negative and positive ESI modes to yield more structure information.Comparison with reference compounds(t_R,MS^n),interpretation of the fragmentation pathways,and searching of in-house database,were utilized for more reliable structure elucidation.A total of 82 components,including 21monoterpene glycosides,four galloyl glucoses,two phenols from PRA,and 55 ginsenosides from GSL,were identified or tentatively characterized from the 70% ethanolic extract of SST.Amongst them,seven and 24 prototype compounds could be detectable in the plasma and urine samples,respectively,after oral administration of an SST extract(4 g×kg^(–1))in rats.No metabolites were observed in the rat samples.The findings of this work first unveiled the chemical complexity of SST and its absorbed components,which would be beneficial to understanding the therapeutic basis and quality control of SST.Shenshao Tablet（SST）,prepared from Paeoniae Radix Alba（PRA）and total ginsenoside of Ginseng Stems and Leaves（GSL）,is a traditional Chinese medicine（TCM）preparation prescribed to treat coronary heart disease.However,its chemical composition and the components that can migrate into blood potentially exerting the therapeutic effects have rarely been elucidated.We developed an HPLC/DAD/ESI-MS^n approach aiming to comprehensively profile and identify both the chemical components of SST and its absorbed ingredients（and metabolites）in rat plasma and urine.Chromatographic separation was performed on an Agilent Eclipse XDB C18 column using acetonitrile/0.1% formic acid as the mobile phase.MS detection was conducted in both negative and positive ESI modes to yield more structure information.Comparison with reference compounds（tR,MS^n）,interpretation of the fragmentation pathways,and searching of in-house database,were utilized for more reliable structure elucidation.A total of 82 components,including 21monoterpene glycosides,four galloyl glucoses,two phenols from PRA,and 55 ginsenosides from GSL,were identified or tentatively characterized from the 70% ethanolic extract of SST.Amongst them,seven and 24 prototype compounds could be detectable in the plasma and urine samples,respectively,after oral administration of an SST extract（4 g×kg^–1）in rats.No metabolites were observed in the rat samples.The findings of this work first unveiled the chemical complexity of SST and its absorbed components,which would be beneficial to understanding the therapeutic basis and quality control of SST.

关 键 词：Shenshao Tablet Paeoniae Radix Alba Total ginsenoside of Ginseng Stems and Leaves Chemical component 

分 类 号：R917[医药卫生—药物分析学]