有氧运动对代谢综合征大鼠氧化应激及心肌PPARα表达的影响  

Influence of aerobic exercise on oxidative stress and PPARα in myocardium in metabolic syndrome rats

作　　者：张崇林[1] 王前进[1] 刘绍生[1] 丁孝民[1] 王世香[1] 王卉[1] Zhang Chonglin;Wang Qianjin;Liu Shaosheng;Ding Xiaomin;Wang Shixiang;Wang Hui(Sports School of Jinggangshan University,Ji＇an 343009,China)

机构地区：[1]井冈山大学体育学院,吉安343009

出　　处：《卫生研究》2018年第5期789-793,797,共6页Journal of Hygiene Research

基　　金：江西省教育厅科技计划项目(No.GJJ150772);江西省教育厅科技计划重点项目(No.GJJ170627);井冈山大学博士科研启动项目(No.JZB11042)

摘　　要：目的观察有氧运动对代谢综合征(MS)大鼠氧化应激反应及心肌过氧化物酶体增生物激活受体α(PPARα)表达的影响。方法 49只4周龄清洁级SD大鼠随机分为空白对照组6只和造模组43只,造模组高脂饲养18周将成模大鼠随机分为模型对照组(MC)8只和模型运动组(ME)8只,ME组跑台有氧训练12周后所有大鼠同期处死,分别测定血清氧化应激因子单核细胞趋化蛋白1(MCP-1)、纤溶酶原激活物抑制物1(PAI-1)、氧化型低密度脂蛋白(ox-LDL)、内皮型一氧化氮合酶(e NOS)及心肌PPARαmRNA表达和蛋白含量。结果与空白对照组比较,模型对照组血清PAI-1、ox-LDL、e NOS显著升高(P<0.05,P<0.01),心肌PPARαmRNA表达和蛋白含量显著降低(P<0.01,P<0.05);与模型对照组比较,ME组血清MCP-1、PAI-1显著降低(P<0.05,P<0.01),心肌PPARαmRNA表达和蛋白含量显著升高(P<0.05,P<0.01)。结论 MS大鼠机体氧化应激反应增强,有氧运动干预能有效降低MS大鼠氧化应激反应,减少氧化应激对心血管的损伤,有氧运动可通过心血管PPARα转录与翻译水平介导了氧化应激反应。Objective This experiment was to investigate the effect of aerobic exercise on oxidative stress response and expression of peroxisome proliferator activated receport α（PPARot） in cardiovascular of metabolic syndrome（MS） rat. Methods 49 4- week male rats were randomly assigned to two groups：control group （C） with 6 rats with normal diet and MS model group with 43 rats with high fat diet. After 18 weeks, assigned the successful MS modeling high fat diet rats to model control group（MC） with 8 rats and model exercise group（ME）with 8 rats. The ME rats exerted aerobic treadmill exercise 12 weeks. Concurrent execution all rats to test the oxidative stress factors in serum including monocyte chemoattractant protein 1 （ MCP-1 ）, plasminogen activator inhibitor 1 （ PAI-1 ）, oxidized low density lipoprotein （ ox-LDL）, endothelial nitric oxide synthase （eNOS） and PPARα mRNA expression and protein content in myocardium. Results Compared to C group, MC group rats PAI-1, ox-LDL, eNOS showed increased in serum （P 〈 0.05 or P 〈0.01） and PPARα mRNA expression and the protein content decreased significantly （P〈0.01, P 〈0.05） in myoeardium. Compare to MC group, ME group rats MCP-1, PAI-1 showed decreased with statistically significant （P 〈 0.05, P 〈 0.01 ） and ox-LDL decreased but without statistical difference in serum and eNOS increased without statistically significant, PPARα mRNA expression increased （P 〈 0.05 ） with the protein content increased with statistically significants（P 〈 0.01 ） in myocardium. Conclusion Oxidative stress increased in MS rats, aerobic exercise could decrease the oxidative stress to reducing the damage of oxidative stress in cardiovascular. The possible mechanism of exercise anti-oxidation stress was the changed PPARc~ transcription and translation by exercise mediated oxidation stress response in cardiovascular.

关 键 词：有氧运动 代谢综合征氧化应激 心血管 过氧化物酶体增生物激活受体α 

分 类 号：R873.01[医药卫生—运动医学] Q591.1[医药卫生—临床医学]