内皮细胞特异性分子-1表达对兔尿源性脓毒血症急性肺损伤的影响  

Dysregulation of endothelial cell specific molecule - 1 expression during urosepsis - induced acute lung injury in rabbits

作　　者：林晓[1] 曾金华 李晓东[2] 许宁[2] 魏勇[2] 吴宇鹏[1] Lin Xiao;Zeng Jinhua;Li Xiaodong;Xu Ning;Wei Yong;Wu Yupeng(Department of Intensive Care Unit,the First Affiliated Hospital of Fujian Medical University,Fuzhou 350005,China（Lin X;Department of Urology,the First Affiliated Hospital of Fujian Medical University,Fuzhou 350005,China（Li XD,Xu N,Wei Y,Wu YP;Mengchao Hepatobiliary Hospital of Fujian Medical University,Fuzhou 350025,China（Zeng JH）Lin Xiao and Zeng Jinhua are the first authors who contributed equally to the article)

机构地区：[1]福建医科大学附属第一医院重症医学科,福州350005 [2]福建医科大学附属第一医院泌尿外科,福州350005 [3]福建医科大学孟超肝胆医院,福州350025

出　　处：《中华实验外科杂志》2018年第10期1817-1820,共4页Chinese Journal of Experimental Surgery

摘　　要：目的观察内皮细胞特异性分子-1（ESM-1）与炎性因子在尿源性脓毒血症导致兔急性肺损伤（ALI）中的表达水平变化，探讨尿源性脓毒血症引起ALI的机制。方法48只健康雄性新西兰兔随机分为对照组、假手术组和模型组。模型组采用肾盂内高压法制备兔尿源性脓毒血症模型。测定3组术后6、12、24、36h共4个时相点，光镜下苏木素．伊红（HE）染色病理切片观察兔肺组织切片病理改变，酶联免疫吸附试验（ELISA）法检测兔血清中肿瘤坏死因子-α（TNF-α）、白细胞介素（IL）-1β、IL-6、ESM-1、淋巴细胞功能相关抗原-1（LFA-1）和细胞间细胞黏附分子-1（ICAM-1）的表达水平。免疫组织化学染色检测家兔肺组织中ESM-1、LFA-1和ICAM-1的表达水平。结果与对照组和假手术组比较，模型组在光镜下观察发现肺组织损伤严重。模型组血清中TNF-α、IL-1β、IL-6的表达明显增加，术后6、12、24、36h表达量分别为TNF-α（63．65±3．07）、（128．17±4．26）、（99．24±7．97）、（49．36±6．09）ng／L；IL-1β：（232．77±21．54）、（481．21±20．84）、（220．23±22．21）、（105．25±23．46）ng／L；IL-6：（72．62±4．42）、（122．51±6．77）、（82．22±4．23）、（60．05±2．82）ng／L；均高于对照组和假手术组相应的各个时段表达量（术后36h，TNF-α：F=96．932，P=0．000；IL-1B：F=9．001，P=0．007；IL-6：F=5．293，P：0．030），模型组血清中LFA-1和ICAM-1随着时间延长表达逐渐增加，术后6、12、24、36h表达量分别为LFA-1：（12．50±5．71）、（23．16±6．84）、（45．04±7．65）、（61．38±9．87）ng／L；ICAM-1：（780．67±66．89）、（1034．16±72．33）、（1420．10±87．25）、（1834．23±88．46）ng／L；与对照组和假手术组相应的各个时段比较差异均有统计学意义（术后36h，LFA．1：F=85．612，P=0．000；ICAM-1：F=456．901Objective To explore the expression of endothelial cell specific molecule - 1 （ ESM - 1 ） and inflammatory factors during urosepsis - induced acute lung injury （ALI ） in rabbits, and the potential mechanism. Methods Urosepsis - induced ALI models in rabbits were assigned into three groups： control group, sham group and urosepsis group （0, 6, 12 and 36 h according to time points pre -scheduled）. The rabbit urosepsis model was established by artificial high pressure in the infected pelvis. Lung histomorphological changes were observed by light microscope. The levels of tumor necrosis factor - α （ TNF - α ）, interleukin （ IL ） - 113, IL - 6, ESM - 1, lymphocyte function associated antigen - 1 （ LFA - 1 ） and intercellular adhesion molecule - 1 （ ICAM - 1 ） in serum were analyzed by enzyme linked immunosorbent assay （ELISA）. The expression of ESM - 1, LFA - 1 and ICAM - 1 in serum was detected by ABC immunohistochemistry, and further the ESM - 1/LFA - 1 rate in lung tissue was calculated. Results As compared with the control group and sham group, urosepsis group had serious lung injury. The levels of serum TNF - a, IL-1β and IL - 6 were elevated significantly in urosepsis group. After 6, 12, 24 and 36 h, the levels of TNF -a were （ 63.65 ± 3.07 ）, （ 128. 17 ± 4. 26 ）, （ 99. 24 ± 7.97 ） , （49. 36 ±6.09） ng/L; the levels of IL-1β were （232. 77 ± 21.54）, （481.21 ± 20. 84）, （220. 23 ± 22. 21）, （105.25 ±23.46） ng/L; the levels of IL-6 were （72.62 ±4.42）, （122.51 ±6.77）, （82. 22 ±4. 23）, （60. 05 ± 2. 82） ng/L. The expression levels in the urosepsis group were significantly higher than those in the control group and the sham group at each time point （ After 36 h, TNF -α ： F = 96. 932, P = 0. 000 ; IL-1β ： F = 9. 001, P = 0. 007 ; IL - 6 ： F = 5. 293, P = 0. 030）. The expression of serum LFA - 1 and ICAM - 1 was increased over time in urosepsis group. After 6, 12, 24 and 36 h, the levelsofLFA-1 were （12.50±5.71）

关 键 词：内皮细胞特异性分子-1 淋巴细胞功能相关抗原-1/细胞间细胞黏附分子-1 尿源性脓毒血症 急性肺损伤 

分 类 号：R392.11[医药卫生—免疫学]