乙二醇诱导果蝇草酸钙结石模型的建立  

Establishment of calcium oxalate calculus model in drosophila induced by ethylene glycol

作　　者：江弘炀 刘卓[1] 刘继红[1] Jiang Hongyang;Liu Zhuo;Liu Jihong(Department of Urology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,Chin;Institute of Urology,Tongfi Hospital,Tongfi Medical College,Huazhong University of Science and Technology,Wuhan 430030,China)

机构地区：[1]华中科技大学同济医学院附属同济医院泌尿外科泌尿外科研究所,武汉430030

出　　处：《中华实验外科杂志》2018年第10期1959-1961,共3页Chinese Journal of Experimental Surgery

摘　　要：目的探索果蝇草酸钙结石模型的培养方法，并对果蝇模型的成功率、适用性进行评价。方法将同天龄雄性黑腹果蝇400只随机分成结石A组（n1=100）、结石B组（n2=100）、结石C组（n3=100）和对照组D组（n4=100），通过二氧化碳麻醉法，分别将其转入带有0．50％、0．75％、1．00％乙二醇的果蝇培养基和不含乙二醇的正常培养基并放入25℃恒温箱内培养。观察统计果蝇的寿命，并解剖果蝇分离体内的马氏管，观察果蝇的结晶形成情况，对形成的晶体进行定性分析。结果在偏光显微镜下观察，A、B、C、D组的果蝇马氏管结晶生成率分别为87．0％、92．0％、98．0％和6．0％（χ2=273．057，P=0．000）。在含0．5％乙二醇培养基（A组）里果蝇中位生存期为36d，0．75％乙二醇培养基（B组）里果蝇中位生存期为31d，1．0％乙二醇培养基（C组）里果蝇中位生存期为23d，正常培养基（D组）的中位生存期为52d，单因素方差分析显示F=35．403，P=0．000。所得晶体通过定性分析为草酸钙晶体。结论0．75％的乙二醇浓度由于其诱导结石形成率高，死亡率相对较低被认为是较理想造模的浓度。稳定的果蝇草酸钙结石模型适合进行高通量的药物试验以及大样本量的结石机制研究。Objective There is not any report on the Drosophila model of calcium oxalate calculus in China yet. We performed this study to develop a Drosophila model of calcium oxalate calculus, and to e- valuate the stability and applicability of the model. Methods 400 same days old male Drosophila melano- gaster were randomly divided into four groups： group A （n1 = 100）, group B （n2 = 100）, group C （n3 = 100） and group D （n4 = 100）. After carbon dioxide anesthesia, they were transferred into conical flask with medium with 0. 50% , 0. 75% , 1.0% ethylene glycol and normal culture medium respectively. Dro- sophila were raised in 25 ~C incubator, and their life span was recorded. After dissection of the Malpighi tubule, the crystal formation of the Drosophila was observed. Then the crystals were obtained and analyzed. Results The crystallization rates of groups A, B, C and D were 87%, 92%, 98% and 6%, respectively （χ2 =273. 057, P =0. 000）. The median life span of group A, B, C, D was 36 days, 31 days, 23 days and 52 days respectively, one - way ANOVA showed that F = 35. 403 and P = 0. 000. The crystals consisted of calcium oxalate. Conclusion 0. 75% of the ethylene glycol concentration is considered to be the ideal concentration due to its high rate of stone formation, the relatively low mortality. In addition, The model is suitable for large sample volume drug test which make big sense for urolithiasis study.

关 键 词：肾结石 果蝇 马氏管 乙二醇 模型 动物 

分 类 号：R691.4[医药卫生—泌尿科学]