结直肠癌患者血清富含半胱氨酸蛋白61的表达及意义  

Expression and significance of serum level of Cry61 in colorectal cancer

作　　者：郑凤萍[1] 罗巨利[1] 王爱华[1] ZHENG Fengping;LUO Juli;WANG Aihua(Department of Anorectal Surgery,the First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410007,China)

机构地区：[1]湖南中医药大学第一附属医院肛肠科,长沙410007

出　　处：《中华实用诊断与治疗杂志》2018年第10期1004-1006,共3页Journal of Chinese Practical Diagnosis and Therapy

基　　金：湖南省科技计划项目(S2012F1023);湖南省科技厅项目(W2012ZT71)

摘　　要：目的探讨结直肠癌患者血清富含半胱氨酸蛋白61(cysteine-rich 61,Cry61)表达水平与其临床病理特征及预后的关系。方法结直肠癌患者160例为观察组,160例体检健康者为对照组,采用ELISA法检测2组血清Cry61水平。绘制ROC曲线,分析血清Cry61对结直肠癌的诊断效能及血清Cry61表达差异与临床病理特征的关系。Kaplan-Meier生存曲线分析血清Cry61的表达差异与临床预后的相关性。多变量Cox回归法分析血清Cry61对预后的影响。结果观察组患者血清Cry61水平[(1 402.1±532.3)ng/L]高于对照组[(604.6±225.1)ng/L](P<0.05);血清Cry61的AUC为0.872(95%CI:0.823～0.910,P<0.001)时,其诊断结直肠癌的灵敏度为77.5%,特异度为95.0%;Cry61>1 357ng/L患者淋巴结转移发生率(43.75%)、血管浸润发生率(52.50%)和TNM分期Ⅲ～Ⅳ期比率(66.25%)均高于Cry61<1 357ng/L患者(25.00%、31.25%、47.50%)(P<0.05);Kaplan-Meier分析显示,Cry61>1 357ng/L患者总生存时间[(501±22)d]低于Cry61<1 357ng/L患者[(801±35)d](P<0.05);单变量Cox回归法显示,血清Cry61>1 357ng/L、淋巴结转移、血管浸润和TNM分期与结直肠癌患者的总生存时间有关(P<0.05);多变量分析显示血清Cry61>1 357ng/L)是结直肠癌患者的独立预后不良因素(HR=3.298,95%CI:1.331～5.115,P=0.015)。结论血清Cry61在结直肠癌患者中表达上调,对结直肠癌诊断和预后评估有一定价值。Objective To investigate the expression of serum level of cysteine-rich 61（Cry61）in patients with colorectal cancer（CRC）and its relationship with the clinicopathological features and prognosis.Methods The serum level of Cry61 was detected by ELISA in 160 patients with CRC（observation group）and 160 healthy volunteers（control group）.ROC curve was drawn to evaluate the diagnostic efficiency of serum Cry61 on CRC.The patients with CRC were divided into high expression group（Cry61〉1 357 ng/L）and low expression group（Cry61〈1 357 ng/L）according to median serum Cry61 of 1 357 ng/L to analyze the relationship between serum Cry61 expression and clinicopathological features.Kaplan-Meier survival curve was used to analyze the correlation between serum Cry61 expression and prognosis.Multivariate Cox regression analysis was used to evaluate the influence of serum Cry61 on prognosis.Results The serum level of Cry61 was significantly higher in observation group（（1 402.1±532.3）ng/L）than that in control group（（604.6±225.1）ng/L）（P〈0.05）.When the AUCof serum Cry61 was 0.872（95%CI：0.823-0.910,P〈0.001）,the sensitivity to CRC was 77.5%,and the specificity was 95.0%.The lymph node metastasis rate（43.75%）,vascular invasion rate（52.50%）and TNM stageⅢ-Ⅳ ratio（66.25%）in high expression group were significantly higher than those in low expression group（25.00%,31.25%,47.50%）（P〈0.05）.Kaplan-Meier analysis showed that the overall survival time was significantly shorter in high expression group（（501±22）d）than that in low expression group（（801±35）d）（P〈0.05）.Univariate Cox regression analysis showed that highly expressed serum Cry61,lymph node metastasis,vascular invasion and TNM staging were correlated with the overall survival time in patients CRC（P〈0.05）.Multivariate Cox regression analysis showed that serum Cry61 was an independent factor for the prognosis of CRC（HR=3.298,95%CI：1.331-5.115,P=0.015）.Conclusion Serum Cry61 is

关 键 词：结直肠癌 富含半胱氨酸61 淋巴结转移 

分 类 号：R735.34[医药卫生—肿瘤]