GDF 11改善老龄鼠内皮依赖性血管舒张功能的实验研究  

Improvement of endothelium-dependent vasodilation in old mice by GDF11

作　　者：刘婷 张承英[2] 苏静克 韩宁[2] 朱彪 LIU Ting;ZHANG Chengying;SU Jingke;HAN Ning;and ZHU Biao(Department of Electrocardiogram,Beijing Nuclear Industry Hospital,Beijing,102413,China;Department of Nephrology,General Hospital of Chinese People＇s Armed Police Force,Beijing 100039,China;Department of Stomatology,Affiliated Hospital of Hebei University,Baoding 071000,China)

机构地区：[1]北京核工业医院心电图室,102413 [2]武警总医院肾内科,北京100039 [3]河北大学附属医院口腔科,保定071000

出　　处：《武警医学》2018年第7期702-705,共4页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的探讨生长分化因子11(growth differentiation factor 11,GDF11)对老龄小鼠内皮依赖性血管舒张功能的影响。方法选择20只18个月龄C57小鼠随机分为实验组(GDF11组,n=10)和对照组(Vehi组,n=10),GDF11组按0.3 mg/(kg·d)的剂量腹腔注射重组GDF11,Vehi组注射等量生理盐水。4周后取材,检测脂代谢相关的血液学指标,分离小鼠的胸主动脉,测定血管舒张功能和主动脉Samd2/3的磷酸化水平。结果与Vehi组比较,GDF11干预显著增强乙酰胆碱(Ach)诱导的内皮依赖性血管舒张功能[10^(-6)mol/L Ach:(37.1±5.2)%vs(59.7±4.3)%;10^(-5)mol/L Ach:(48.2±4.0)%vs(78.4±5.6)%;10^(-4)mol/L Ach:(61.1±4.7)%vs(90.2±7.4)%],降低血清中三酰甘油的水平[(76.6±11.2)vs(64.5±10.4)mg/dl],升高脂联素的浓度[(11.9±1.1)vs(14.2±1.7)μg/ml],并能提高主动脉Samd2/3的磷酸化水平[Smad2:(1.00±0.05)vs(1.78±0.10)]、Smad3[(1.00±0.09)vs(1.57±0.12)]。结论 GDF11对血管内皮细胞有保护作用。Objective To investigate the effects of GDF11 on endothelium-dependent vasodilation of the aorta in old mice.Methods Twenty 18-month-old C57 mice were randomly divided into GDF11 group [0. 3 mg/（ kg·d） ] recombinant GDF11 intraperitoneal injection,n = 10） and Vehi group（ equivalent physiological saline injection,n = 10）. After four weeks of intervention,blood biomarkers of lipid metabolism were tested,while relaxation response and Western blot were determined in the isolated aorta.Results GDF11 intervention significantly increased endothelium-dependent vasodilation in response to acetylcholine（ Ach）[10^-6 mol/L Ach：（ 37. 1 ± 5. 2） % vs（ 59. 7 ± 4. 3） % 、10^-5 mol/L Ach：（ 48. 2 ± 4. 0） % vs（ 78. 4 ± 5. 6） % 、10^-4 mol/L Ach：（ 61. 1 ± 4. 7） % vs（ 90. 2 ± 7. 4） %],decreased triglyceride levels in serum [（ 76. 6 ± 11. 2） vs（ 64. 5 ± 10. 4） mg/dl],elevated adiponectin protein levels [（ 11. 9 ± 1. 1） vs（ 14. 2 ± 1. 7） μg/ml] and enhanced protein levels of phosphorylated Samd2/3[p-Smad2：（ 1. 00 ± 0. 05） vs（ 1. 78 ± 0. 10） ]、p-Smad3 [（ 1. 00 ± 0. 09） vs（ 1. 57 ± 0. 12） ]. Conclusions GDF11 can attenuate impaired endothelium-dependent vasodilation in old mice.

关 键 词：GDF11 内皮依赖性血管舒张功能 老龄鼠 

分 类 号：R543.1[医药卫生—心血管疾病]