辽宁地区猪伪狂犬病毒病原学检测与遗传演化分析  

Pathogenic detection and genetic analysis of porcine pseudorabies virus in Liaoning province

作　　者：杨洺扬 杨本勇[1] 申贯男[1] 关淼[1] 李井春[1] 梁乔[1] 张健[1] 李蓉[1] 王宏燕[1] 杨国丽[1] Yang Mingyang;Yang Benyong;Shen Guannan;Guan Miao;Li Jingchun;Liang Qiao;Zhang Jian;Li Rong;Wang Hongyan;Yang Guoli(Liaoning center for animal disease control and prevention,Liaoning Shenyang 110164)

机构地区：[1]辽宁省动物疫病预防控制中心,辽宁沈阳110164

出　　处：《现代畜牧兽医》2018年第10期51-56,共6页Modern Journal of Animal Husbandry and Veterinary Medicine

摘　　要：本研究利用实时荧光定量PCR方法筛选伪狂犬病毒阳性样品,建立PCR方法特异性扩增阳性毒株LNP-1株gD、gE基因,并进行测序及遗传演化分析。结果表明LNP-1株gD、gE基因与近年来我国流行毒株遗传关系接近。通过基因序列比对发现,LNP-1株gD基因与经典疫苗毒株Bartha株gD基因相比在831-836位之间存在连续6个碱基插入,并有多个A-G替换,gE基因在第142～144位和1 488～1 490位分别存在GAC和CGA碱基插入,符合近年国内伪狂犬变异毒株gE基因突变特征,推断其为伪狂犬变异毒株。从而为判断辽宁地区流行伪狂犬毒株类型,伪狂犬净化和疫苗选择提供参考。In this study, pseudorabies positive samples were screened by real-time fluorescent quantitative PCR, the gD and gE genes of LNP-1 were amplified by PCR method, sequenced, and ana‐lyzed. The results showed that the gD and gE genes of LNP-1 had a close relationship with the ep‐idemic strain in our country in recent years. Through genetic sequence alignment, found that com‐pared with classical Bartha vaccine strains, LNP-1 gD gene existed an insertion row in 831～835 which was six bases, and some A-G substitution. In gE gene GAC and CGA inserted into 142～144 and 1488-1490, that conformed to the domestic pseudorabies mutant strain gE gene mutation character‐istics in recent years, inferred to be a pseudorabies mutant strain. It could provide the refer‐ence to judge the epidemic pseudrabies virus types in Liaoning province, purification pseudora‐bies and selection vaccine.

关 键 词：猪伪狂犬病毒 GD基因 GE基因 检测 遗传演化分析 

分 类 号：S858.28[农业科学—临床兽医学]