改良终点法在临床生化检验中的效果  

The effect of modified end point method in clinical biochemistry test

作　　者：张卫荣 ZHANG Wei-rong(Department of clinical laboratory,Haimen Third People＇s Hospital,Nantong Jiangsu,226151)

机构地区：[1]海门市第三人民医院检验科,江苏南通226151

出　　处：《牡丹江医学院学报》2018年第5期64-65,134,共3页Journal of Mudanjiang Medical University

摘　　要：目的探讨改良终点法在临床生化检验中的效果。方法将2016年1月至2017年3月间在我院接受生化检验的60例患者纳入课题研究,评价改良终点法与原终点法的相关性、精密度和线性回归分析。结果改良终点法与原终点法的临床检测结果无显著差异,具有一定的相关性;正常浓度下,改良终点法与原终点法测定的批内CV和总CV无显著差异,P>0. 05;异常浓度下,改良终点法测定的批内CV和总CV均小于2. 6%,而原终点法测定的总CV大于2. 6%,两种测定方法存在统计学差异,P <0. 05;改良终点法的线性回归方程为:y=6. 127x-2. 519,r=0. 9983;原终点法的线性回归方程为:y=4.374x+0. 536,r=0. 9787。结论改良终点法与原终点法在中、低浓度的线性关系均较好,但是相比于原终点法,改良终点法的线性浓度更宽,有利于降低试剂用量,节约成本,值得在临床上广泛推广使用。Objective To analyze the effect of modified end point method in clinical biochemistry test. Methods 60 patients received clinical biochemistry test were selected. Correlation analysis,precision,and linear regression analysis of original end point method and modified end point method were compared. Results There was no significant difference of the test results between original end point method and modified end point method,and the two methods had certain correlation,P 〉 0. 05; In detecting samples of normal concentration,the CV of the two methods were all lower than 2. 60%,the difference was not significant,P 〉 0. 05; There was significant difference of the test results detecting samples of abnormal concentration between original end point method（ CV 〈 2. 60%） and modified end point method（ CV 〉 2. 60%）,P 〈 0. 05; The test regression equation of original end point method： y = 4. 374 x ＋ 0. 536,r = 0. 9787; The test regression equation of modified end point method： y = 6. 127 x-2. 519,r = 0. 9983. Conclusion Modified end point method has well linear scope in clinical biochemistry test.

关 键 词：原终点法 改良终点法 生化检验 效果 

分 类 号：R446.1[医药卫生—诊断学]