‘冲天桃’NBS类抗病基因同源序列的克隆与分析  

Cloning and Analysis of NBS Type Resistance Gene Analogs in 'Chongtian Peach'(Prunus Persica L. Batch)

作　　者：杨春雷[1] 卜璐璐[1] 张世杰[1] 李春艳[1] 罗兴会 张鸿昌 黄国嫣 刘艳 知史 杨正安[1] 杨荣萍[1] Yang Chunlei;Bu Lulu;Zhang Shijie;Li Chunyan;Luo Xinghui;Zhang Hongchang;Huang Guoy-an;Liu Yan;Zhi Shi;Yang Zhengan;Yang Rongping(Yunnan Agricultural University,Kunming,650201;Gardening Station of Weishan County Agricultural Bureau,Dali,671000;Agricultural Com-prehensive Service Center of Tao Yuan Town,Yongsheng County,Lijiang,674100;The Apple Industry Research Institute in Zhaotong,Zhaotong,657000;The Fruit and Vegetable Technology Extension Station in Mengzi,Mengzi,661100;Plant Protection and Quarantine Station of Diqing Ti-betan Autonomous Prefecture,Diqing,674400)

机构地区：[1]云南农业大学,昆明650201 [2]巍山县农业局园艺站,大理671000 [3]永胜县涛源镇农业综合服务中心,丽江674205 [4]昭通苹果产业研究所,昭通657000 [5]蒙自果蔬技术推广站,蒙自661100 [6]迪庆藏族自治州植保植检站,迪庆674400

出　　处：《分子植物育种》2018年第20期6591-6597,共7页Molecular Plant Breeding

基　　金：云南省高水平大学园艺学创新人才培养基地建设项目(云教高(2015)57号);云南农业大学学生科技创新创业行动基金(2016ZKX060)共同资助

摘　　要：云南地方桃资源丰富,冲天桃为其中一种,为了寻找云南省地方桃资源中具有高抗病的种质资源,从而为云南省抗病性地方桃品种选育提供理论基础,本研究根据已知植物抗病基因的NBS-LRR类保守序列设计简并引物,以冲天桃为试材,对其基因组DNA进行PCR扩增、克隆、测序,共得到5条NBS类抗病基因同源序列(RGAs),大小约为500 bp,经序列分析表明:这5条RGAs与桃亚属植物中推测的一些抗病基因蛋白具有较高的同源性,其中一些RGAs与番茄抗青枯病基因Prf及烟草的抗花叶病毒N基因具有高度的同源性。因此可以确定‘冲天桃’植株中存在较为丰富的NBS-LRR类抗病基因,这为下一步云南省抗病性地方桃品种选育提供了研究依据。There are rich local peach resources in Yurman, and ＇chongtian peach＇ is one of them. In order to find the germplasm resources with high resistance to disease in the local peach resources in Yunnan Province and provide a theoretical basis for the breeding of disease-resistant local peach varieties in Ytmnan Province, in this study, the design of degenerate primers was based on the NBS-LRR class conservative sequence of known plant resistance genes. Taking ＇chongtian peach＇ as a test material, PCR amplification, cloning and sequencing of its genomic DNA were conducted. A total of 5 NBS class resistant gene analogs （RGAs） were obtained, and the size was about 500 bp. Sequence analysis showed that these 5 RGAs were highly homologous to some of the disease-resistant proteins in the plants of peach subgenus. Some of the RGAs genes were highly homologous to the gene Prf against bacterial wilt of tomato and the N gene of tobacco mosaic virus. Therefore, it could be confirmed that there were abundent NBS-LRR class resistance genes in the plants of Prunus persica, which might lay the foundation for further breeding of disease-resistant local peach varieties in Yunnan Province.

关 键 词：冲天桃 NBS抗病基因 同源序列 克隆与分析 

分 类 号：S662.1[农业科学—果树学] Q943.2[农业科学—园艺学]