蒺藜苜蓿TCP转录因子家族的全基因组鉴定和分析  

Genome-wide Identification and Analysis of the TCP Transcription Factor Family of Medicago truncatula

作　　者：李菲[1] 何小红[1] 张宇斌[1] 乙引[1] Li Fei;He Xiaohong;Zhang Yubin;Yi Yin(Key Laboratory of Plant Physiology and Development Regulation of Guizhou Province,School of Life Science,Guizhou Normal University,Guiyang,55002)

机构地区：[1]贵州师范大学生命科学学院植物生理与发育调控贵州省重点实验室,贵阳550025

出　　处：《分子植物育种》2018年第20期6639-6645,共7页Molecular Plant Breeding

基　　金：贵州省科学技术基金(2017GZ66249)资助

摘　　要：作为植物特有的转录因子家族,TCP基因处于植物多种信号传导途径的中心节点位置。对蒺藜苜蓿全基因组进行检索,共获得21个TCP基因序列。蒺藜苜蓿TCP成员间在蛋白质大小、等电点等方面具有明显的差异。对蒺藜苜蓿TCP基因进行基因结构、染色体定位和系统进化分析,发现蒺藜苜蓿TCP基因结构较为简单,一般不含有内含子,仅一个外显子。21个基因中仅有5个基因有内含子。系统进化关系上其中4个基因亲缘关系非常近。这4个基因位于1号、4号、7号染色体上TCP基因聚集存在的位置。蒺藜苜蓿TCP基因在各个器官间特异性的表达。TCP基因参与蒺藜苜蓿根瘤发育的过程,也响应外界盐胁迫和病菌侵染刺激。本研究发现的蒺藜苜蓿TCP基因家族信息,可为蒺藜苜蓿乃至豆科植物育种提供有价值的信息。As a plant-specific transcription factor tamlly, the IUF genes lm at me centra~ nuuc pontoon ux ua~ plant＇s multiple signaling pathways. A total of 21 TCP genes order were obtained by the retrieval of the whole genome of M. truncatula. There were significant differences in protein size, isoelectric point, etc. between TCP members of Medicago truncatula. The gene structure, chromosomal location and phylogenetic analysis of the TCP genes ofM. truncatula revealed that the structure of the TCP genes ofM. truncatula was simple without intron but with only one exon. Of the 21 genes, only 5 genes had one intron. The phylogenetic relationship of four genes was very close. These four genes are located on chromosomes 1, 4, and 7, where TCP gene aggregates. TCP genes of M. trur^cat^la express differently in each different organs. TCP genes are involved in the process of root development of M. truncatula, and in the response to external salt stress and pathogen infection. The TCP gene family information of M. truncatula was found in this study which might provide valuable information for the breeding ofM. truncatula and leguminous plants.

关 键 词：蒺藜苜蓿 TCPs基因家族 进化 表达模式 

分 类 号：Q943.2[生物学—植物学] S541.9[农业科学—作物学]