烤烟苗期烟碱含量与其代谢基因的相关性分析  

Analysis on the Correlation between Nicotine Content and Metabolizing Genes in Flue-cured Tobacco Seedling Stage

作　　者：尹鹏嘉[1,2] 周世奇 任民[1] 孙鑫 罗成刚[1] 杨爱国[1] 张玉[1] 陈爱国[1] 李晓旭[1] 文柳璎[1] Yin Pengjia;Zhou Shiqi;Ren Min;Sun Xin;Luo Chenggang;Yang Aiguo;Zhang Yu;Chen Aiguo;Li Xiaoxu;Wen Liuying(Tobacco Research Institute of Chinese Academy of Agricultural Sciences,Key Open Laboratory of Tobacco Genetic Improvement and Biotechnolo-gy,Qingdao,266101;Graduate School of Chinese Academy of Agriculture Sciences,CAAS,Beijing,100081;The Office for the Protection of New Varieties of Plants,Beijing,100081)

机构地区：[1]中国农业科学院烟草研究所中国农业科学院烟草遗传改良与生物技术重点开放实验室,青岛266101 [2]中国农业科学院研究生院,北京100081 [3]农业部植物新品种保护办公室,北京100081

出　　处：《分子植物育种》2018年第20期6753-6760,共8页Molecular Plant Breeding

基　　金：中国农业科学院科技创新工程(ASTIP-TRIC01);中国农业科学院烟草研究所青年科学基金(2015A01)共同资助;中央级公益性科研院所基本科研业务费专项(161023201-6015);山东省自然科学基金(ZR2016CB39)

摘　　要：为了在苗期对高烟碱的烤烟种质进行分子选育提供参考。采用水培技术在中国农业科学院烟草研究所人工气候室对7份高低烟碱种质的烤烟进行种植,并在苗期对其叶部烟碱含量及烟碱合成、转运基因以及囊泡运输调节因子的表达量进行检测和分析。结果表明:苗期7个烤烟品种之间烟碱含量变异丰富,QRT-PCR结果表明,在苗期与烟碱代谢相关的基因NtMATE1/NtMATE2、NtPMT在叶部不表达,NtNUP1在叶部表达较弱,而QRT-PCR结果表明,在烟草苗期与烟碱代谢相关基因在不同高低烟碱种质之间的表达差异无明显规律。此外,主成分分析表明,苗期根部2个烟碱合成基因(NtPMT, NtQPT)以及囊泡运输调节因子(GEF)是影响叶部烟碱含量的主要因子,MATE1/MATE2、JAT1是苗期根部转运烟碱的主要蛋白。而在苗期叶部中JAT是主要烟碱转运蛋白,NtQPT基因也是影响烟碱含量的主要因子;烟碱与其代谢基因的网络关系图显示,根部NtPMT基因是影响叶部烟碱含量的主要因子,NtMATE1/NtMATE2基因的表达对2个烟碱合成基因的表达有促进作用。In order to provide reference for molecular breeding of high nicotine flue-cured tobacco germplasm at seedling stage. Seven varieties of flue-cured tobacco with high and low nicotine were planted in Tobacco Research Institute of Chinese Academy of Agricultural Sciences by hydroponics technology. The nicotine content in leaves, nicotine biosynthetic, transport genes and the expression of vesicular transport regulatory factors were detected and analyzed at seedling stage. The results showed that the nicotine content of 7 flue-cured tobacco varieties were abundant at seedling stage. QRT-PCR results showed that the genes NtMA TE1/NtMA TE2 and NtPMT related to nicotine metabolism were not expressed in leaves, while NtNUP1 was weakly expressed in leaves at seedling stage. But QRT-PCR results showed that there was no obvious difference in the expression of genes related to nicotine metabolism between different high and low nicotine germplasms at tobacco seedling stages. In addition, the principal component analysis showed that the two nicotine synthesis genes （NtPMT, NtQPT） and vesicular traffic regulator factor （GEF） were the main factors affecting the nicotine content in leaves at the seedling stage, and MATE1/MATE2 and JAT1 were the main proteins transporting nicotine in roots at the seedling stage. What eles, the JAT1 was the major protein of nicotine transport in the leaves of the seedling stage, and the NtQPT gene in leaves was also the main factor affecting nicotine content; the network diagram of nicotine and nicotine metabolic genes showed that NtPMT gene in roots was the main factor that affected the nicotine content in leaves. The expression ofNtMA TE1/NtMA TE2 gene promoted the expression of two nicotine biosynthetic genes.

关 键 词：烤烟 烟碱 相关性 苗期 

分 类 号：S572[农业科学—烟草工业]