炎症因子在新生缺氧缺血性脑病大鼠脑组织中的表达及意义  

Expression and significance of inflammatory factors in brain tissues of neonatal rats with hypoxic-ischemic encephalopathy

作　　者：曾志涌 卢顺玉 黎云鹏 邸伟 ZENG Zhiyong;LU Shunyu;LI Yunpeng;DI Wei(Department of Pediatrics,Longgang District Second People＇s Hospital,Shenzhen 518112,China;Department of Pharmacy,Liuzhou Worker＇s Hospital in Guangxi Zhuang Autonomous Region,Liuzhou 545005,China;Department of Cerebral Surgery,Ningdu County People＇s Hospital in Jiangxi Province,Ningdu 342800,China)

机构地区：[1]深圳市龙岗区第二人民医院儿科,广东深圳518112 [2]广西壮族自治区柳州市工人医院药剂科,广西柳州545005 [3]江西省宁都县人民医院脑外科,江西宁都342800

出　　处：《中国现代医生》2018年第28期38-41,共4页China Modern Doctor

基　　金：国家自然科学基金项目(81500965);广东省深圳市科技计划项目(JCYJ20150402092653974)

摘　　要：目的探讨炎症因子在新生儿缺氧缺血性脑病(hypoxic-ischemic encephalopathy,HIE)大鼠脑组织中的表达及意义。方法 36只新生SD大鼠,随机分为对照组、缺血缺氧0 h、6 h、1 d、3 d、7 d组,每组6只。观察各组大鼠脑组织中TNF-α、CXCL10、CXCL12、 IL-1α、IL-1β、IL-1R mRNA表达差异。结果 TNF-α在HIE发生后开始增高,6～24 h达到高峰后逐渐下降,72 h仍高于正常,而后下降,但仍高于对照组,至7 d后呈现低表达。CXCL10/12在0 h时表达与对照组对比未见差异,在6 h及12 h达到高峰,至72 h逐渐下降但仍高于对照组,至7 d时基本降至正常。相对对照组,IL-1R在整个HIE过程中未见明显高表达,而在疾病的初期及恢复期其发病早期却出乎意料的呈现低表达的特点(0 h、24 h、72 h),至7 d后恢复正常。IL-1α、IL-1β在0 h时期呈现低表达,6 h呈现高表达,而后在24 h后恢复正常,至7 d后IL-1α复又呈现低表达趋势。结论炎症因子与HIE发病进展密切相关,并且在疾病的不同进程中各种炎症因子起着不同的作用,可以作为判断疾病进展的客观指标。Objective To investigate the expression and significance of inflammatory factors in the brain tissues of neonatal rats with hypoxic-ischemic encephalopathy（HIE）. Methods 36 newborn SD rats were randomly divided into control group, and hypoxic-ischemic 0 h, 6 h, 1 d, 3 d, 7 d groups, with 6 rats in each group. The differences in the expressions of TNF-α, CXCL10, CXCL12, IL-1α, IL-1β and IL-1R mRNA were observed in the brain tissues of rats in each group. Results TNF-α was increased after HIE, peaked at 6-24 h and was then decreased, and was still higher than normal at 72 h, and was then decreased. However, it was still higher than the control group and showed low expression after 7 d. There was no difference in CXCL10/12 expression at 0 h compared with the control group, peaked at 6 h and 12 h, and was gradually decreased at 72 h but still higher than the control group. It was basically decreased to normal at 7 d. Compared with the control group, IL-1R did not show significant high expression during the whole HIE process. In the early stage of the disease and in the stage of recovery, it unexpectedly showed low expression （0 h, 24 h, 72 h） at the early stage of onset, and returned to normal after 7 d. IL-1α and IL-1β showed low expression at 0 h but turned to high expression at 6 h, and then returned to normal after 24 h, but IL-1α showed a low expression trend after 7 d. Conclusion Inflammatory factors are closely related to the progression of HIE, and various inflammatory factors play different roles in different processes of the disease, which can be used as an objective indicator to judge disease progression.

关 键 词：新生儿缺血缺氧性脑病 TNF-Α CXCL 白细胞介素 

分 类 号：R722.1[医药卫生—儿科]