miR-137通过PTN抑制瘢痕成纤维细胞生长  

Inhibition of MiR-137 on the growth of hypertrophic scar fibroblasts through PTN

作　　者：王洪一[1] 林时秀[1] 马书丹 徐志山 陶凯[1] WANG Hong-yi;LIN Shi-xiu;MA Shu-dan;XU Zhi-shan;TAO Kai(Reconstructive and Plastic Surgery,The General Hospital of Shenyang Military Region,Shenyang 110840,China)

机构地区：[1]沈阳军区总医院整形外科,辽宁沈阳110840

出　　处：《中国美容整形外科杂志》2018年第10期599-602,J0003,共5页Chinese Journal of Aesthetic and Plastic Surgery

摘　　要：目的分析miR-137对瘢痕成纤维细胞生长的影响。方法通过芯片分析技术筛选出增生性瘢痕差异性表达的miRNAs,并在增生性瘢痕组织中进行验证。通过miRDB软件预测miR-137能够打靶的蛋白质,采用荧光素酶报告基因实验分析miR-137对PTN的靶向作用。提取原代瘢痕成纤维细胞分别构建miR-137过表达或低表达的细胞系。采用western blot及real time PCR检测miR-137对细胞中PTN的影响,以及MTT方法检测miR-137对细胞增殖的作用;检测miR-137对细胞中Cyclin B1的影响。结果 miR-137在增生性瘢痕组织中呈低表达,可以与PTN的3′UTR区域直接结合来抑制其表达及活性,从而抑制瘢痕成纤维细胞的增殖。Western blot和real time PCR检测结果显示,miR-137能抑制Cyclin B1在瘢痕成纤维细胞中的表达。结论 miR-137可以抑制PTN的表达,而且可以通过抑制PTN来抑制瘢痕成纤维细胞的生长。Objective To analyze the effect of miR-137 on the growth of hypertrophic scar fibroblasts. Methods The differentially expressed miRNAs in hypertrophic scars were screened by microarray analysis, and confirmed in hypertrophic scar tissue. Proteins that miR-137 targeted were predicted through miRDB software, and the targeting effect of miR-137 on PTN was analyzed by luciferase reporter gene. Primary hypertrophic scar fibroblasts were extracted to construct the cell lines with overexpression or low expression of miR-137. The effect of miR-137 on cell proliferation was detected by MTT, and the effect on Cyclin B1 was further detected by western blot and real time PCR. Results There was low Mi R-137 expression in hypertrophic scar tissue. The expression and activity of miR-137 can be inhibited by directly combining with the 3′UTR region of PTN, thus inhibiting the proliferation of hypertrophic scar fibroblasts. The results of western blot and real time PCR showed that miR-137 can inhibit the expression of Cyclin B1 in scar fibroblasts.Conclusion MiR-137 can inhibit the expression of PTN and thereby inhibit the growth of scar fibroblasts.

关 键 词：增生性瘢痕 miR-137 PTN 增殖 

分 类 号：R622[医药卫生—整形外科]