新型超微量冷冻麦管：微量精子冻融安全有效新载体(附83例报道)  

A novel micro-straw(LSL straw): the reliable cryopreservation vehicle for rare or very low concentrations of human sperm(83 cases reported)

作　　者：薛云婧 徐源 刘锋[1] 朱子珏[1] 公跃华 汪小波 胡剑麟 孙健[2] 陈威[2] 张箴波[2] 洪艳[1] 吴煜[2] 李铮[1] Xue Yunjing;Xu Yuan;Liu Feng;Zhu Zijue;Gong Yuehua;Wang Xiaobo;Hu Jianlin;Sun Jian;Chen Wei;Zhang Zhenbo;Wu Yu;Li Zheng(Department of Andrology,Center for Men＇s Health,Department of ART,Institute of Urology,Urologic Medical Center,Shanghai General Hospital,Shanghai Key Lab of Reproductive Medicine,Shanghai Jiao Tong University,Shanghai 200080,China)

机构地区：[1]上海交通大学附属第一人民医院泌尿外科中心男科,上海交通大学泌尿外科研究所男性健康评估中心,上海市生殖医学重点实验室,200080 [2]上海交通大学附属第一人民医院辅助生殖医学科,200080

出　　处：《中华生殖与避孕杂志》2018年第9期718-723,共6页Chinese Journal of Reproduction and Contraception

基　　金：上海市新兴前沿技术(上海申康医院发展中心SHDC12015122); 上海交通大学医工交叉重点项目(上海交通大学医学院YG2017ZD04); 上海市科学技术委员会(17JC1420403)

摘　　要：目的探讨新型超微量冷冻麦管(LSL管)冻存微量精子的冻融结果。方法回顾性分析83例严重男性不育症患者采用LSL管冻存精子的冻融结果,观察其中23对夫妇因男性因素解冻LSL管精子行卵胞质内单精子显微注射(intracytoplasmic sperm injection,ICSI)的妊娠结局。结果不同来源的微量精子采用LSL管复苏后89.5%(94/105)可见活动精子,进入辅助生殖周期的标本可使用率为73.9%(17/23)。在18个ICSI周期中,受精率、卵裂率、可用胚胎率和优质胚胎率分别为78.0%、92.5%、62.7%和40.6%;种植率、临床妊娠率和累计活产率分别为31.1%、52.5%和55.6%。获得的所有12个活产儿均未见出生缺陷。结论 LSL管是一种可用于微量精子冷冻保存的安全有效新载体,可广泛使用于辅助生殖治疗。Objective To analyze the pregnancy outcome of cases who underwent intracytoplasmic sperm injection（ICSI） with frozen-thawed sperm, which was rare and weak, cryopreserved by a novel microstraw（LSL micro-straw）. Methods The present study retrospectively analyzed the post-thaw results of cryopreserved human sperm from 83 severe infertility male patients（including oligospermia and asthenospermia）. Then we investigated the evolution of embryo development and pregnancy outcome in 23 patients underwent ICSI. Results About 89.5%（94/105） frozen-thawed samples could observe motile sperm in quality control. About 73.9%（17/23） sperm were available in 18 ICSI cycles. The fertilization rate, the cleavage rate, the available embryo rate and the high-quality embryo rate were 78.0%, 92.5%, 62.7% and 78.0%, respectively. The implantation rate, the clinical pregnancy rate and the cumulative live birth rate were 31.1%, 52.5% and 55.6%,

关 键 词：微量精子 冻融 新型超微量冷冻麦管(LSL管) 卵胞质内单精子显微注射(ICSI) 妊娠结局 

分 类 号：R714.8[医药卫生—妇产科学]