偶氮氯膦Ⅲ探针吸收光谱法测定药物中的卡马西平  

Determination of carbamazepine in drugs by absorption spectrometry with chlorophosphonazo Ⅲ as a probe

作　　者：张淑琼[1] 李娜 程丹 江虹[1] ZHANG Shu-qiong;LI Na;CHENG Dan;JIANG Hong(College of Chemistry and Chemical Engineering,Collaborative Innovation Center for Green Development in Wuling Mountain Areas,Yangtze Normal University,Chongqing 408100,China)

机构地区：[1]长江师范学院化学化工学院,长江师范学院武陵山片区绿色发展协同创新中心,重庆408100

出　　处：《现代化工》2018年第10期240-243,共4页Modern Chemical Industry

基　　金：重庆市教委科技基金资助项目(KJ1401226); 长江师范学院科技基金资助项目(2017CXX158)

摘　　要：建立了简便、快速、准确测定卡马西平的单波长和双波长可见吸收光谱法,探讨了反应条件、共存物质的影响及光谱特征。在弱酸性溶液中,偶氮氯膦Ⅲ与卡马西平以静电引力结合生成具有2个正吸收峰的离子缔合物,最大和次大正吸收波长分别为614 nm和666 nm,在此2个波长处,卡马西平的质量浓度在0~4.7 mg/L范围内与吸光度A呈线性关系,服从朗伯-比尔定律,表观摩尔吸光系数(κ)分别为1.58×10~4L/(mol·cm)(614 nm)和1.20×10~4L/(mol·cm)(666 nm)。用双波长叠加法测定时,其表观摩尔吸光系数(κ)达2.78×10~4L/(mol·cm),方法的回收率为98.0%~102%,相对标准偏差RSD(n=5)为2.3%~2.7%。该方法可用于药片中卡马西平的测定。A simple,rapid and accurate new method for the determination of carbamazepine by single wavelength and dual-wavelength visible absorption spectra is developed.The influences brought by reaction conditions and the coexisting substances,and the spectral characteristics are discussed.In a weak acidic solution,chlorophosphonazo Ⅲcombines with carbamazepine via electrostatic attraction to form an ionic association complex with two positive absorption peaks.The maximum and secondary positive absorption wavelengths are located at 614 nm and 666 nm,respectively.At these two wavelengths,the mass concentration of carbazepine keeps linear with absorbance A in the range of 0 to 4.7 mg·L^-1,which obeys Lambert-Beer law.The apparent molar absorptivities（κ） are 1.58×10^4 L/（mol·cm）（614 nm） and1.20×10^4 L/（mol·cm）（666 nm） respectively.When the dual-wavelength superposition absorption spectrophotometry is used to determine the concentrations of carbamazepine,its apparent molar absorptivity（κ） can achieve 2.78 × 10^4 L/（mol·cm）.The method＇s recovery and RSD（n= 5） are in the ranges of 98.0%-102% and 2.3%-2.7% respectively.The method can be used for the determination of carbamazepine in tablets with satisfactory results.

关 键 词：卡马西平 偶氮氯膦Ⅲ 吸收光谱 单波长 双波长 

分 类 号：O657.3[理学—分析化学] R917[理学—化学]