壳聚糖支架负载SHH和CM-Dil标记的脂肪干细胞移植后存活分化研究  

Experimental Research of Chitosan Scaffold Loaded with SHH and CM-Dil Labeled Adipose Derived Stem Cells Survival Differentiation after Transplantation

作　　者：罗鸣骜 伍尚敏[1] 苏晓三[1] 高嘉 于璐[1] 王翼寅 甘凤山 LUO Ming-ao;WU Shang-min;SU Xiao-san;GAO Jia;YU Lu;WANG Yi-yin;GAN Feng-shan(Department of Plastic Surgery,the First People＇s Hospital of Kunming,Kunming 650000,Yunnan,China)

机构地区：[1]昆明市第一人民医院整形外科,云南昆明650000

出　　处：《中国美容医学》2018年第10期129-132,共4页Chinese Journal of Aesthetic Medicine

基　　金：昆明市科技局科技成果推广应用及科技惠民计划重点项目(项目编号:昆科计字2016-1-S-06455)

摘　　要：目的:观察壳聚糖支架负载音猬因子(Sonic hedgehog,SHH)和脂肪干细胞(adipose-derived stem cells,ADSCs)移植后,ADSCs存活分化的效果。方法:将健康C57BL/6小鼠随机分为A组(PBS+ADSCs)、B组(SHH+ADSCs)、C组(壳聚糖+ADSCs)、D组(壳聚糖+SHH+ADSCs)。将各组混合物分别注射到小鼠背部皮下。术后1、2、4、8周处死小鼠,通过对移植组织一般观察、荧光显微镜观察、HE染色、免疫组化染色来评价移植细胞存活分化的情况。结果:术后8周,壳聚糖支架完全降解,大体观察、HE染色、免疫组化染色均说明壳聚糖支架同时搭载SHH和ADSCs脂肪干细胞存活率较高,新生血管增加,效果均优于A、B、C三组。结论:壳聚糖支架同时负载SHH和ADSCs可有效促进干细胞存活,显著增加血管组织数量。Objective The aim of our research is to observe the survival and differentiation of adipose Derived stem cells after transplantation with injectable chitosan scaffold loaded with SHH and adipose stem cells. Methods Thirty healthy C57BL/6 mice were selected. Random division group A mixture of PBS and ADSCs,group B mixture of SHH and ADSCs,group C chitosan scaffold loaded with ADSCs,group D chitosan scaffold loaded with SHH and ADSCs.After 1,2,4 and 8 weeks analysis involved general observation,HE staining,immunohistochemical staining of the survival and differentiation of transplanted cells. Results Eight weeks after operation, chitosan scaffold were completely degraded. After 8 weeks,analysis involved general observation,HE staining and immunofuorescence staining showed that Chitosan scaffold loaded with SHH and ADSCs could effectively increase the survival rate of adipose stem cells,increase angiogenesis. All the results were better than that of A、B、C group. Conclusion Chitosan scaffold loaded with SHH and ADSCs can effectively promote the survival of stem cells and spontaneously differentiate into mature adipocytes and increase the number of vascular tissues.May serve as a promising strategy for improving the survival of ADSCs graft survival rate.

关 键 词：可注射性 壳聚糖 音猬因子 脂肪干细胞 干细胞移植 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]