CD100在正常皮肤、急性创面及慢性创面中的表达研究  

Expression of CD100 in Normal Tissue, Acute Wound and Chronic Wound

作　　者：刘蓓[1,2] 王芳 陈琳[2] 余州 宋雅娟 王彤 苏映军 LIU Bei;WANG Fang;CHEN Lin;YU Zhou;SONG Ya-juan;WANG Tong;SU Ying-jun(Department of Medical Technology;Department of Medical Cosmetology,the First Affliated Hospital,Xi＇an Medical University,Xi＇an 710021,Shaanxi,China;Department of Plastic and Reconstructive Surgery,Xijing Hospital,the Fourth Military Medical University,Xi＇an 710032,Shaanxi,China)

机构地区：[1]西安医学院医学技术系,陕西西安710021 [2]空军军医大学第一附属医院整形外科,陕西西安710032 [3]西安医学院第一附属医院医学美容科,陕西西安710021

出　　处：《中国美容医学》2018年第10期133-136,共4页Chinese Journal of Aesthetic Medicine

基　　金：国家自然科学基金青年项目(81401598):Semaphorin4D/CD100调节糖尿病慢性创面愈合的作用及机制

摘　　要：目的:研究CD100分子在正常皮肤组织、急性创面及慢性创面中的表达。方法:采用免疫组化法检测外科手术切除的20例正常皮肤组织、30例急性创面及20例慢性创面边缘全层皮肤组织CD100的表达水平。结果:正常皮肤CD100主要表达于表皮,其中胞膜高表达,真皮不表达;急性创面中表皮CD100膜表达水平显著降低;慢性创面中CD100在表皮组织低表达而真皮表达显著升高。各组间表皮胞膜、胞质及真皮中CD100表达水平比较,差异有统计学意义(P<0.05)。结论:在皮肤组织损伤早期,表皮组织膜型CD100的脱落可能对创面愈合早期发挥重要作用,慢性创面CD100表皮持续性低表达、真皮高表达的状态可能和创面难愈有关。Objective To investigate the expression of CD100 in normal skin tissue, acute wound and chronic wound tissues. Methods Immunohistochemical method was used to detect the expression of CD100 in 20 cases of normal skin tissues, 30 cases of acute wound and 20 cases of chronic wound tissues. Results The expression of CD100 in normal skin tissues was mainly expressed in the epidermis, and was not expressed in dermis. The expression level of membrane CD100 of epidermis in acute wound was signifcantly reduced. In the chronic wound, the expression level of CD100 in the epidermis was low, but the expression level in the dermis was increased signifcantly. The expression levels of CD100 in membrane and cytoplasm of epidermal and in dermis had statistical difference in the three groups （P〈0.05）. Conclusion In the early skin tissue damage, shedding of membrane CD100 in epidermis may play an important role in acute wound healing. In chronic wounds, the condition of low CD100 expression in epidermis and the high CD100 expression in epidermis may be related to the diffculty of chronic wound healing.

关 键 词：创面愈合 CD100 免疫组织化学法 急性创面 慢性创面 

分 类 号：R64[医药卫生—外科学]