纳米TiO_2对斑马鱼超氧化物歧化酶活性的影响  

Effects of Titanium Dioxide Nanoparticles on SOD Activity in Zebrafish

作　　者：张章 唐文浩 唐天乐 ZHANG Zhang;TANG Wenhao;TANG Tianle(Institute of Tropical Agricuhure and Forestry,Hainan University,Haikou 570228,China;School of Tropical and Laboratory Medicine,Hainan Medical University,Haikou 571199,China)

机构地区：[1]海南大学热带农林学院,海口570228 [2]海南医学院热带医学与检验医学院,海口571199

出　　处：《热带生物学报》2018年第3期274-280,共7页Journal of Tropical Biology

基　　金：海南省自然科学基金面上项目(318MS060);中西部高校提升综合实力工作资金资助项目(ZXBJHXK004)

摘　　要：利用斑马鱼成鱼,研究金红型纳米TiO_2(Titanium dioxide nanoparticles,TiO_2NPs)对斑马鱼的氧化损伤效应。以超氧化物歧化酶(Superoxide Dismutase,SOD)作为生物标志分子,考察斑马鱼成鱼染毒10 d后,鳃、肝脏和肠3种组织内SOD水平的变化,通过酶联免疫吸附反应(ELISA)和荧光定量PCR(Q-PCR),对SOD在蛋白和分子水平的变化进行检测。结果表明:TiO_2NPs会导致斑马鱼鳃和肝脏组织内SOD活性的降低,但对肠组织内SOD的活性影响不大;在基因水平上,TiO_2NPs会导致sod基因表达上调2～3倍。由此推论,TiO_2NPs长期暴露会影响斑马鱼体内的SOD水平,导致鳃和肝脏组织的氧化损伤。An attempt was made to observe the effect of rutile titanium dioxide nanoparticles （TiO 2 NPs） on oxidative damage of zebrafish （Danio rerio） .Adult zebrafish was exposed to TiO 2 NPs at the concentrations of 10,50 and 100 mg·L -1 for 10 days with the exposure to the bulk TiO 2 （100 mg·L -1 ） as the control.Twenty adult zebrafish were treated at each concentration,and six fish treated were caught to analyze their changes of superoxide dismutase （SOD） activities in the gills,livers and guts.The SOD was used as a bimarker,and the SOD changes at the protein and molecular level were analyzed by using the enzyme-linked immunosorbent assay （ELISA） and real-time quantitative PCR（Q-PCR）.Results showed that the TiO 2 NPs decreased the SOD activity in the gill and liver tissues of zebrafish,but had little effect on the SOD activity in the gut tissue.The sod gene expression was up-regulated by 2-3 times in the three tissues.It is concluded that long-term exposure of zebrafish to TiO 2 NPs can give an impact on the SOD level within the body of zebrafish and cause oxidative damage to gills and livers of zebrafish.

关 键 词：TiO2NP8 斑马鱼 超氧化物歧化酶 酶联免疫吸附反应 实时荧光定量PCR 

分 类 号：Q55[生物学—生物化学]