火龙果干旱胁迫下根和茎的基因差异表达  

Differentially Expressed Genes of Roots and Stems of Pitaya under Drought Stress

作　　者：齐钊[1] 闫臻[1] 徐敏[1] 刘成立 程玉[1] 熊睿[1] 骆娟[1] 汤华[1] QI Zhao;YAN Zhen;XU Min;LIU Chengli;CHENG Yu;XIONG Rui;LUO Juan;TANG Hua(Hainan Key Laboratory of Sustainable Utilization of Tropical Biological Resources/Institute of Tropical Agriculture and Forestry,Haikou,Hianan 570228,China)

机构地区：[1]海南大学海南省热带生物资源可持续利用重点实验室/热带农林学院,海口570228

出　　处：《热带生物学报》2018年第3期320-327,共8页Journal of Tropical Biology

基　　金：海南省重点研发项目(ZDYF2018080);国家自然科学基金资助(31360364)

摘　　要：以‘大红’火龙果品种为遗传材料,通过RNA-Seq测序技术,对干旱组与对照组火龙果的根和茎进行转录组测序,筛选出与干旱胁迫相关的差异表达基因,并进行qRT-PCR验证。结果表明:从火龙果根系中筛选到194个干旱胁迫下的差异表达基因,包括70个上调表达基因和124个下调表达基因;从火龙果茎中筛选到21个干旱胁迫下的差异表达基因,包括12个上调表达基因和9个下调表达基因。筛选到的差异表达基因主要富集在酪氨酸代谢、次生代谢生物合成、糖酵解/糖异生、碳代谢等过程,表明火龙果主要是通过调节自身基础代谢来适应干旱胁迫,其中,酪氨酸代谢很可能在火龙果抗旱过程中起重要作用。Although pitaya （Hylocereus undatus） has a good drought tolerance, drought stress could result in re- duced production, low quality, and even failure in bearing fruit of pitaya. The pitaya Dahong was used as the experimental material, and a new generation of high-throughput sequencing technology （RNA-Seq） was used to compare the differences in the root and stem of the pitaya between the treatment group and the control group. We screened differentially expressed genes （DEGs） from the root and stem, and verified the expression of the screened genes by qRT-PCR. A total of 194 DEGs were produced from CR-VS-DR, including 70 up-regulated expression genes and 124 down-regulated expression genes which responded to drought stress. From CS-VS-DS, 21 DEGs were obtained, including 12 up-regulated expression genes and 9 down-regulated expression genes which responded to drought stress. The sereened differentially expressed genes were mainly concentrated in the process of tyrosine metabolism, secondary metabolite biosynthesis, glycolysis/glycoheterosis, and carbon metabo- lism. It showed that the pitaya was mainly adapted to drought stress by adjusting its basic metabolism, and the tyrosine metabolism may play an important role in the drought resistance of the pitaya.

关 键 词：火龙果 干旱胁迫 转录组测序 差异表达基因 

分 类 号：Q786[生物学—分子生物学]