前列腺素E1抗大鼠骨髓间充质干细胞凋亡的最佳浓度探讨  

The optimal concentration of prostaglandin E1 against apoptosis of rat bone marrow mesenchymal stem cells

作　　者：刘丛勇 张露 苏峰 邓保平[1] 曾宽[1] 杨艳旗[1] LIU Congyong; ZHANG Lu; SU Feng; DENG Baoping; ZENG Kuan; YANG Yanqi(Department of Cardiac Surgery, Sun Yatsen Memorial Hospital, Sun Yatsen University, Guangzhou, 510120, China)

机构地区：[1]中山大学孙逸仙纪念医院心脏外科,广州510120

出　　处：《岭南现代临床外科》2018年第5期504-508,513,共6页Lingnan Modern Clinics in Surgery

基　　金：国家自然科学基金青年科学基金项目(81600245);广东省科技计划项目(2013B021800102);中山大学百人计划(F002009011)

摘　　要：目的探讨在体外条件下前列腺素E1抗大鼠骨髓间充质干细胞(BMSCs)凋亡的最佳浓度。方法提取SD大鼠骨髓间充质干细胞,取P3-P5代的大鼠骨髓间充质干细胞在体外缺血清缺氧条件下进行培养,将前列腺素E1分别以0μg/L(对照组)、1μg/L、10μg/L、20μg/L、30μg/L、40μg/L、50μg/L、100μg/L的浓度作用于大鼠骨髓间充质干细胞,并设置48 h,72 h二个时间点,用流式细胞检测的方法测定每个时间点及每个浓度组的大鼠骨髓间充质干细胞凋亡率,最后对数据进行统计分析。结果在各个时间点中,质量浓度为1μg/L、10μg/L、20μg/L、30μg/L、40μg/L、50μg/L、100μg/L的前列腺素E1均可减少大鼠骨髓间充质干细胞的凋亡,其中20μg/L浓度组的大鼠骨髓间充质干细胞凋亡率最低;前列腺素E1在作用48 h后,大鼠骨髓间充质干细胞凋亡率最低。结论体外缺血清缺氧条件下,前列腺素E1抗大鼠骨髓间充质干细胞凋亡的最佳浓度是20μg/L,且其抗凋亡作用在48 h时达到高峰。Objective To explore the optimal concentration of prostaglandin E1 against apoptosis of rat bone marrow mesenchymal stem cells in vitro. Methods Bone marrow mesenchymal stem cells were extracted from SD rats, rat bone marrow mesenchymal stem cells （P3-P5） w-ere cultured in hypoxia/ serum-deprivation （H/SD） model, in vitro, and Prostaglandin E 1 take different concentrations （0μg/L, 1 μg/L, 10 μg/L, 20 μg/L, 30 μg/L, 40 μg/L, 50 μg/L, 100 μg/L ）, anset 48 h, 72 h two groups, then measure the apoptosis rate of bone marrow- mesenchymal stem cells in each group by flow- cytometiT and analyze the data by statistical methods. Results The apoptosis rate of bone marrow mesenchymal stem cells could be reduced by Prostaglandin E1 at concentrations of 1, 10, 20, 30, 40, 50 and 100 μg/L, the lowest apoptosis rate was at 20 μg/L; Rat bone marrow mesenchymal stem cells have the lowest apoptosis rate at 48 hours. Conclusion The optimal concentration of prostaglandin E1 against apoptosis of rat bone marrow mesenchymal stem cells was 20 μg/L, and its anti-apoptotic effect peaked at 48 h in vitro.

关 键 词：前列腺素E1 骨髓间充质干细胞 凋亡 浓度 

分 类 号：R654[医药卫生—外科学]