核酸检测与血清学检测在输血传染病筛查中的应用  

Application of Nucleic Acid Testing and Serological Testing in Pre-transfusion Test Santao

作　　者：张叁涛 彭华丽 段元山 马杰 ZHANG San-tao;PENG Hua-li;DUAN Yuan-shan(Clinical laboratory of Hubei Provincial Hospital of Integrated Chinese ＆ WesternMedicine,Wu Han 430015)

机构地区：[1]湖北省中西医结合医院检验科,武汉430015

出　　处：《临床输血与检验》2018年第6期578-581,共4页Journal of Clinical Transfusion and Laboratory Medicine

摘　　要：目的对比分析输血前感染指标的核酸检测和血清学检测结果,探讨相关检测的临床意义。方法采用酶联免疫吸附试验检测本院2016年4月1日～2017年4月1日共4177例接受输血前检查的患者血清中感染性指标(抗-HCV、抗-HIV、抗-TP、HBsAg、HBsAb、HBeAg、HBeAb、HBcAb),采用核酸检测技术对其中1509例患者血清中HBVDNA和HCVRNA进行检测,并结合患者年龄、性别对这些结果进行统计和分析。结果 4177例患者中,检出HBsAg阳性患者376例(9%)、抗-HCV阳性患者54例(1.29%)、抗-TP阳性患者118例(2.82%)、抗-HIV阳性患者6例(0.14%)。进行核酸检测的1509例患者中,HBsAg阳性者的HBVDNA阳性率为55.56%,HBsAg阴性而HBVDNA阳性者所占比例为0.22%,抗-HCV抗体阳性者的HCVRNA阳性率为80.00%。结论对拟输血患者进行核酸检测和血清学检测,有利于相关感染性疾病的发现与诊断,也有利于减少因输血和侵入性操作引起感染的发生。Objective To analyze the result of pre-transfusion test and discuss the significance of relative test.Method A total of 4 177 cases of patients who come to our hospital for therapy on April 1,2016 to April 1,2017 wereenrolled. With ELISA or PCR,we detected the serum infectious disease indicators,and statistically analyzed the resultsassociated with age and sex. Result Among the patients we delineated,the positive rate of HBsAg,anti-HCV,anti-TP and anti- HIV were 9%,1.29%,2.82%,and 0.14%. The positive rate of HBV DNA among HBsAg positive patients is 55.56%.The positive rate of HBV DNA among HBsAg negative patients is 0.22%.The positive rate of HCV RNA among anti-HCVpositive patients is 80%. Conclusion Nucleic acid testing and serological testing are necessary for patients who may acceptblood transfusion,relative test is meaningful in reducing infection of transfusion.

关 键 词：输血 传染病 核酸检测 血清学检测 

分 类 号：R457.1[医药卫生—治疗学] R446.11[医药卫生—临床医学]