高渗盐水对大鼠脑损伤后脑水肿区水通道蛋白4及caspase-3表达的影响  

Effects of hypertonic saline on expressions of aquaporin 4 and caspase-3 in brain edema area after traumatic brain injury in rats

作　　者：尹剑[1] 金许洪[1] 吕庆平 陈怀 庞晓俊 魏雨雨 Yin Jian, Jin Xuhong, Lyu Qingping, Chen Huai, Pang Xiaojun, Wei Yuyu.(Department of Neurosurgery, Hangzhou Red Cross Hospital, Hangzhou 310003, China)

机构地区：[1]浙江省中西医结合医院神经外科,杭州310030

出　　处：《中华创伤杂志》2018年第10期953-958,共6页Chinese Journal of Trauma

基　　金：杭州市科学技术委员会资助项目（20150633B28）

摘　　要：目的探讨高渗盐水对大鼠创伤性脑损伤（TBI）后脑水肿区水通道蛋白4（AQP4）和caspase-3表达的影响。 方法选取sD雄性大鼠72只，体重220-250 g，按随机数字表法分为假手术组（A组）、脑挫伤＋等渗盐水组（B组）和脑挫伤＋高渗盐水组（C组），每组24只。采用Feeney自由落体撞击法建立中度TBI大鼠模型，并分别给予等渗盐水和高渗盐水进行干预。伤后6，24，48 h行神经功能学评分；取脑组织测脑组织含水量，伊文思蓝染色检测血脑屏障稳定情况；免疫组化法测定AQP4阳性细胞表达情况；Western blot检测脑组织中AQP4及caspase-3表达水平；TUNEL法检测脑组织神经元细胞凋亡情况。 结果 与A组比较，B组神经功能学评分有所下降，而脑组织含水量、伊文思蓝含量、AQP4（6 h1．73±0．31：0．33±0．13；24 h：2．47±0．27：0．33±0．14；48 h：2．18±0．19：0．33±0．12）及caspase-3水平（6 h：0．53±0．18：0．34±0．07；24 h：0．58±0．16：0．33±0．08；48 h：0．59±0．11：0．33±0．07）、TUNEL阳性细胞表达明显升高（P均〈0．05）。与B组同时相点比较，C组神经功能学评分明显提高，脑组织含水量明显减少。伊文思蓝含量明显下降，AQP4（6 h：1．51±0．27：1．73±0．31；24 h：2．13±0．13：2．47 ±0．27；48 h：1．84±0．22：2．18 4-0．19）及easpase-3（6 h：0．44±0．09：0．53±0．18；24 h：0．46±0．10：0．58±0．16；48 h：0．48±0．12：0.59±0．11）水平下调，TUNEL阳性细胞表达明显减少（P均〈0．05）。结论高渗盐水通过下调AQP4和easpase-3，可减少神经细胞凋亡，减轻大鼠TBI后脑水肿，明显改善神经功能症状。Objective To investigate the effect of hypertonic saline on the expressions of aquaporin 4 （AQP4） and caspase-3 in the brain edema area after traumatic brain injury （TBI） in rats Methods Seventy-two male SD rats weighing 220-250 g were selected and randomly divided into three groups （24 rats per group） ： sham operation group （Group A）, traumatic brain injury ± normal saline group （Group B） and traumatic brain injury ± hypertonic saline group （Group C ）. Moderate TBI model was induced by Feeney＇s free falling method. Normal saline and hypertonic saline were delivered respectively. The neurological score was measured at 6, 24, and 48 hours after operation. The brain water content was measured, and the blood brain barrier stability was detected by Evans blue staining. AQP4 positive cells was detected by immunohistoehemistry. The expressions of AQP4 and caspase-3 protein in brain tissue were detected by Western blot, and the apoptosis of neurons in brain tissue by TUNEL method. Results Compared with Group A, the neurological score of Group B were obviously decreased, while the water content in the brain tissue, Evans blue staining, AQP4 positive ceils, AQP4 （6 hours： 1.73 ±0.31 vs. 0.33±0.13;24hours： 2. 47±0. 27 vs. O. 33±0. 14;48 hours： 2.18± 0. 19 vs. 0. 33 ±0.12） , caspase-3 protein expression（6 hours： 0.53 ±0.18 vs. 0. 34 ：t：0.07 ;24 hours： 0.58±0.16 vs. 0.33 ±0.08;48 hours： 0.59±0.11 vs. 0.33 ±0.07） and apoptosis index in brain tissue in Group B were significantly increased （ all P 〈 0.05 ）. Compared with Group B, the neurological score of Group C were obviously increased, while the water content in the brain tissue, Evans blue staining, AQP4 positive cells, AQP4 （6 hours： 1.51 ±0.27 vs. 1.73 ±0.31 ;24 hours： 2.13 ±0.13 vs. 2.47 ± 0. 27 ;48 hours： 1.84 ± 0.22 vs. 2. 18 ± 0. 19） and Caspase-3 protein expression （ 6 hours： 0.44±0.09vs. 0.53 ±0. 18;24 hours： 0.46±0. 10 vs. 0.58 ±0. 16;48 hours： 0.48 ±0. 12 vs. 0.59 �

关 键 词：脑损伤 盐水 高渗 脑水肿 水通道蛋白质4 caspase-3 

分 类 号：R651.15[医药卫生—外科学]